Chen Qingxin, Cheng Ke, Wang Wanhe, Yang Liu, Xie Yusheng, Feng Ling, Zhang Jie, Zhang Huatang, Sun Hongyan
Department of Chemistry and Center of Super-Diamond and Advanced Films, City University of Hong Kong, Hong Kong, China.
Key Laboratory of Biochip Technology, Biotech and Health Centre, City University of Hong Kong Shenzhen Research Institute, Shenzhen, 518057, China.
J Pharm Anal. 2020 Oct;10(5):490-497. doi: 10.1016/j.jpha.2020.07.003. Epub 2020 Aug 4.
Hydrogen peroxide (HO) plays a significant role in regulating a variety of biological processes. Dysregulation of HO can lead to various diseases. Although numerous fluorescent imaging probes for HO have been reported, the development of HO ratiometric fluorescent probe with large Stokes shift remains rather limited. Such probes have shown distinct advantages, such as minimized interference from environment and improved signal-to noise ratio. In this work, we reported a new pyrene-based compound as HO fluorescent probe in vitro. The probe demonstrated ratiometric detection behavior, large Stokes shift and large emission shift. In addition, the probe showed high sensitivity and selectivity towards HO in vitro. Based on these excellent properties, we successfully applied to the visualization of exogenous and endogenous HO in living cells. Cell imaging study also showed that our probe was localized in the mitochondria. We envision that the probe can provide a useful tool for unmasking the biological roles of mitochondrial HO in living systems.
过氧化氢（HO）在调节多种生物过程中发挥着重要作用。HO调节异常会导致各种疾病。尽管已经报道了许多用于HO的荧光成像探针，但具有大斯托克斯位移的HO比率荧光探针的开发仍然相当有限。这类探针具有明显的优势，如环境干扰最小化和信噪比提高。在这项工作中，我们报道了一种新型芘基化合物作为体外HO荧光探针。该探针表现出比率检测行为、大斯托克斯位移和大发射位移。此外，该探针在体外对HO表现出高灵敏度和选择性。基于这些优异性能，我们成功地将其应用于活细胞中外源和内源HO的可视化。细胞成像研究还表明，我们的探针定位于线粒体。我们设想该探针可为揭示线粒体HO在生命系统中的生物学作用提供有用工具。
