Univ. Estadual Paulista - UNESP, Department of Biology, Laboratory of Microscopy and Microanalysis, Cristóvão Colombo St., 2265, São José do Rio Preto, São Paulo, Brazil.
Department of Structural and Functional Biology, State University of Campinas, Bertrand Russel Av., Campinas, São Paulo, Brazil.
Exp Mol Pathol. 2018 Aug;105(1):130-138. doi: 10.1016/j.yexmp.2018.07.003. Epub 2018 Jul 9.
Telocytes are recently categorised CD34-positive interstitial cells that comprise the cells which were previously called interstitial Cajal-like cells (ICLCs). These were detected in the stroma of various organs such as the prostate, lungs, mammary glands, liver, gallbladder, and jejunum, among others. Several functions have been proposed for telocytes, such as a supportive role in smooth muscle contraction and immune function in adult organs, and tissue organisation and paracrine signalling during development, as well as others. In the jejunum, little is known about the function of telocytes in the adult organ, or is there any information about when these cells develop or if they could have an auxiliary role in the development of the jejunum. The present study employed histological, immunohistochemical and immunofluorescence techniques on histological sections of the jejunum of Mongolian gerbil pups on two different days of postnatal development of the jejunum, covering the maturation period of the organ. By immunolabelling for CD34, it was observed that telocytes are already present in the jejunum during the first week of postnatal life and exist in close association with the developing muscularis mucosae, which are therefore TGFβ1-positive. The telocytes are still present at the end of the first month of life, and a portion of them present co-localisation with c-Kit. Fibroblast-like cells, which are exclusively c-Kit-positive, are also observed, which may indicate the presence of interstitial Cajal cells (ICCs). Finally, it can be hypothesised that a portion of the telocytes may give rise to ICCs, which are c-Kit-positive but CD34 negative.
间质细胞最近被归类为 CD34 阳性的间质细胞，它们包含以前称为间质 Cajal 样细胞（ICLCs）的细胞。这些细胞在各种器官的基质中被检测到，如前列腺、肺、乳腺、肝、胆囊和空肠等。已经提出了几种间质细胞的功能，例如在平滑肌收缩和成年器官的免疫功能中的支持作用，以及在发育过程中的组织组织和旁分泌信号传导等。在空肠中，对于成年器官中间质细胞的功能知之甚少，也没有关于这些细胞何时发育或它们是否可以在空肠发育中发挥辅助作用的信息。本研究在蒙古沙鼠幼仔空肠的两个不同出生后发育天数的组织切片上采用组织学、免疫组织化学和免疫荧光技术，涵盖了器官的成熟阶段。通过对 CD34 的免疫标记，观察到间质细胞在出生后第一周的空肠中已经存在，并与发育中的黏膜肌层密切相关，因此 TGFβ1 阳性。在生命的第一个月结束时，这些细胞仍然存在，其中一部分与 c-Kit 共定位。还观察到仅 c-Kit 阳性的成纤维细胞样细胞，这可能表明存在间质 Cajal 细胞（ICCs）。最后，可以假设一部分间质细胞可能会产生 ICCs，这些细胞 c-Kit 阳性但 CD34 阴性。
