Department of Structural and Functional Biology, State University of Campinas, Campinas, São Paulo, Brazil.
Department of Biology, Univ. Estadual Paulista (UNESP), São José do Rio Preto, São Paulo, Brazil.
J Cell Mol Med. 2017 Dec;21(12):3309-3321. doi: 10.1111/jcmm.13234. Epub 2017 Aug 24.
Telocytes are CD34-positive interstitial cells, known to exert several functions, one of which is a role in tissue organisation, previously demonstrated by telocytes in the myocardium. The existence of telocytes in the prostate has recently been reported, however, there is a lack of information regarding the function of these cells in prostate tissue, and information regarding the possible role of these cells in prostatic development. This study used immunofluorescence techniques in prostate tissue and prostatic telocytes in culture to determine the relationship between telocytes and prostate morphogenesis. Furthermore, immunofluorescent labelling of telocytes was performed on prostate tissue at different stages of early postnatal development. Initially, CD34-positive cells are found at the periphery of the developing alveoli, later in the same region, c-kit-positive cells and cells positive for both factors are verified and CD34-positive cells were predominantly observed in the interalveolar stroma and the region surrounding the periductal smooth muscle. Fluorescence assays also demonstrated that telocytes secrete TGF-β1 and are ER-Beta (ERβ) positive. The results suggest that telocytes play a changing role during development, initially supporting the differentiation of periductal and perialveolar smooth muscle, and later, producing dense networks that separate alveoli groups and form a barrier between the interalveolar region and periurethral smooth muscle. We conclude that telocytes play a relevant role in prostate tissue organisation during postnatal development.
间质细胞是 CD34 阳性的间质细胞，已知具有多种功能，其中之一是在组织组织中发挥作用，这一作用先前已在心肌中的间质细胞中得到证实。前列腺中的间质细胞的存在最近已经被报道，然而，关于这些细胞在前列腺组织中的功能的信息仍然缺乏，并且关于这些细胞在前列腺发育中的可能作用的信息也缺乏。本研究使用免疫荧光技术在前列腺组织和培养的前列腺间质细胞中确定了间质细胞与前列腺形态发生之间的关系。此外，还在早期产后发育的不同阶段的前列腺组织上进行了间质细胞的免疫荧光标记。最初，在发育中的肺泡的外围发现 CD34 阳性细胞，后来在同一区域中，验证了 c-kit 阳性细胞和两种因子均为阳性的细胞，并且 CD34 阳性细胞主要存在于肺泡间基质和围绕导管周围平滑肌的区域中。荧光分析还表明，间质细胞分泌 TGF-β1 并且是 ER-Beta（ERβ）阳性的。结果表明，间质细胞在发育过程中起着变化的作用，最初支持导管周围和肺泡周围平滑肌的分化，后来产生密集的网络，将肺泡群分开，并在肺泡间区域和尿道周围平滑肌之间形成屏障。我们得出结论，间质细胞在产后发育过程中的前列腺组织组织中起着重要作用。
