Beijing Chaoyang Hospital, 100020, The Worker Stadium South Road No.8, Chaoyang district, Beijing, China.
Beijing Huairou Hospital, 101400, No. 9 North Street, Yongtai, Huairou District, Beijing, China.
Life Sci. 2018 Sep 1;208:46-54. doi: 10.1016/j.lfs.2018.07.014. Epub 2018 Jul 9.
Hypoxia makes cells death but it is not always true. Short term anoxia can boost remaining cells activities and promote tissue repair. This study is to explore the effects of hypoxic preconditioning (HP) on osteoblasts and analyze the involved lncRNA and mRNA.
Human osteoblast was used. Normoxic control group (N) were cultured in normoxic culture medium (L-DMEM, 10% FBS and 1% penicillin-streptomycin) 8 h. Long-term hypoxic group (H) were cultured in 4 h hypoxic culture medium (200 μmol/L CoCl and L-DMEM)/4 h normoxic culture medium. Hypoxic preconditioning group (HP) were received 3 times HP (cultured in 10 min hypoxic culture medium/10 min normoxic culture medium, which was HP 1times) and then cultured in 4 h hypoxic culture medium/4 h normoxic culture medium. Cell proliferation ability, osteonectin, transforming growth factor-β1, insulin like growth factors-1 and osteopontin were test. The lncRNA and mRNA was detected by high-throughput sequencing. p-Value < 0.05 was accepted as statistically significant.
Cell proliferation activity: N > HP > P. Corrected osteonectin, TGF-β1, IGF-1 and osteopontin: HP > P > N. High-throughput sequencing of gene shown the expression of 4630 RNAs. Further, we identified that HP group made 239 and 160 target genes up-regulated and down-regulated respectively compared with H group (p < 0.05). GO, KEGG and PPI analysis indicated relevant biological processes.
Hypoxic preconditioning alleviated cells anoxia tolerance and promoted cells to synthesis many cytokines. The protection mechanism involved many lncRNAs and mRNAs.
缺氧会导致细胞死亡，但情况并非总是如此。短期缺氧可以增强剩余细胞的活性并促进组织修复。本研究旨在探讨缺氧预处理（HP）对成骨细胞的影响，并分析相关的长链非编码 RNA（lncRNA）和信使 RNA（mRNA）。
使用人成骨细胞。常氧对照组（N）在常氧培养基（L-DMEM、10%胎牛血清和 1%青霉素-链霉素）中培养 8 小时。长期缺氧组（H）在 4 小时缺氧培养基（200μmol/L CoCl 和 L-DMEM）/4 小时常氧培养基中培养。缺氧预处理组（HP）接受 3 次 HP（在 10 分钟缺氧培养基/10 分钟常氧培养基中培养，这是 HP 1 次），然后在 4 小时缺氧培养基/4 小时常氧培养基中培养。检测细胞增殖能力、骨粘连蛋白、转化生长因子-β1、胰岛素样生长因子-1 和骨桥蛋白。通过高通量测序检测 lncRNA 和 mRNA。p 值<0.05 为有统计学意义。
细胞增殖活性：N>HP>HP。校正后的骨粘连蛋白、TGF-β1、IGF-1 和骨桥蛋白：HP>HP>N。基因的高通量测序显示了 4630 个 RNA 的表达。此外，与 H 组相比，HP 组有 239 个和 160 个靶基因分别上调和下调（p<0.05）。GO、KEGG 和 PPI 分析表明了相关的生物学过程。
缺氧预处理减轻了细胞缺氧耐受能力，并促进了细胞合成多种细胞因子。保护机制涉及许多 lncRNA 和 mRNA。
