Department of Orthopedics, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China.
Biomed Res Int. 2019 Apr 16;2019:6592464. doi: 10.1155/2019/6592464. eCollection 2019.
To find a simple and noninvasive method to promote fracture healing, we are trying to explore whether repetitive brief ischemia would promote bone healing. 88 rats divided into 6 groups were used to make right tibia closed fracture caused by the heavy weight collision method. Healthy side groups received homemade tourniquet placed on left and affected side group placed on right thigh 10 min inflated/10 min deflated 3 times every 24 hours or 48 hours after tibia fractured. Rats in control groups received homemade tourniquet uninflated placed on right thigh 1 hour every 24 hours or 48 hours. X-rays were checked at 1, 2, and 4 weeks. Micro-CT inspected the bone healing at 2 and 4 weeks. Serum cytokines, such as bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF), diethanolamine enzyme activity unit of alkaline phosphatase (ALP) and transforming growth factor-1 (TGF-1), interleukin 10 (IL-10) and interleukin 6 (IL-6), were checked at 1, 2, and 4 weeks. Local histology was evaluated at 2 weeks. HE dye and BMP-2, VEGF, TGF-, and ALP immunohistochemical stains were made. Callus areas of posterior-anterior and lateral views were calculated and repetitive brief ischemia increased the callus areas ratio at 1 and 2 weeks. Besides, from micro-CT results, repetitive brief ischemia increased the bone volume (BV) at 2 and 4 weeks and also increased the total bone tissue volume (TV) at 2 weeks and BV/TV at 4 weeks. The serum cytokines, such as BMP-2, VEGF, diethanolamine enzyme activity unit of ALP and TGF-1, have increased by repetitive brief ischemia at 1, 2 weeks. It is opposite of affected side group that the level of serum IL-10 increased and IL-6 decreased in healthy side group at 1, 2 weeks. Repetitive brief ischemia increased the callus area at 2 weeks and boosted the synthesis of BMP-2, VEGF, TGF-, and ALP in the fracture region at 2 weeks from tissue stains. Repetitive brief ischemia promotes bone healing no matter on the affected side or the healthy side limb.
为了寻找一种简单、无创的方法来促进骨折愈合，我们试图探索重复短暂性缺血是否会促进骨愈合。使用 88 只大鼠，通过重物撞击的方法造成右侧胫骨闭合性骨折，将其分为 6 组。健康侧组在左侧和患侧组在右侧大腿使用自制止血带，每 24 小时充气/放气 3 次，每次 10 分钟，在胫骨骨折后即刻开始。对照组每 24 小时在右侧大腿使用自制未充气的止血带 1 小时。在第 1、2、4 周进行 X 射线检查。在第 2 和 4 周使用 micro-CT 检查骨愈合情况。在第 1、2 和 4 周检测血清细胞因子，如骨形态发生蛋白-2（BMP-2）、血管内皮生长因子（VEGF）、二乙醇胺酶活性单位碱性磷酸酶（ALP）和转化生长因子-1（TGF-1）、白细胞介素 10（IL-10）和白细胞介素 6（IL-6）。在第 2 周进行局部组织学评估。进行 HE 染色和 BMP-2、VEGF、TGF-和 ALP 免疫组织化学染色。计算前后位和侧位的骨痂面积，重复短暂性缺血可增加 1 周和 2 周时的骨痂面积比。此外，从 micro-CT 结果来看，重复短暂性缺血可增加 2 周和 4 周时的骨体积（BV），并可增加 2 周时的总骨组织体积（TV）和 4 周时的 BV/TV。重复短暂性缺血可增加血清细胞因子，如 BMP-2、VEGF、ALP 的二乙醇胺酶活性单位和 TGF-1，在 1 周和 2 周时增加。在健康侧组中，与患侧组相反，血清 IL-10 在 1 周和 2 周时增加，IL-6 减少。重复短暂性缺血可增加 2 周时的骨痂面积，并从组织染色中增加 2 周时骨折区域 BMP-2、VEGF、TGF-和 ALP 的合成。重复短暂性缺血无论在患侧还是健侧肢体均能促进骨愈合。
