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转录组分析候选基因与. 香气形成相关

Transcriptomic Analysis of the Candidate Genes Related to Aroma Formation in .

机构信息

College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China.

College of Forestry, Nanjing Forestry University, Nanjing 210037, China.

出版信息

Molecules. 2018 Jul 2;23(7):1604. doi: 10.3390/molecules23071604.

Abstract

, or "RiXiangGui", is an ornamental, woody, evergreen plant that is cultivated widely because it blooms recurrently and emits a strong fragrance. Recently, the germplasm resources, classification, and aroma compositions of . have been investigated. However, the molecular mechanisms of the floral scent formation and regulation have remained largely unknown. To obtain a global perspective on the molecular mechanism of the aroma formation during blooming, nine RNA Sequencing (RNA-Seq) libraries were constructed from three flowering stages: The initial, full, and final flowering stage. In short, a total of 523,961,310 high-quality clean reads were assembled into 136,611unigenes, with an average sequence length of 792 bp. About 47.43% of the unigenes (64,795) could be annotated in the NCBI non-redundant protein database. A number of candidate genes were identified in the terpenoid metabolic pathways and 1327 transcription factors (TFs), which showed differential expression patterns among the floral scent formation stages, were also identified, especially , , , and , which could play critical roles in the floral scent formation. These results indicated that the floral scent formation of . was a very complex process which involved a large number of TFs. This study provides reliable resources for further studies of the floral scent formation.

摘要

瑞香,是一种观赏、木质、常绿植物,由于其反复开花且散发浓郁香味而被广泛种植。最近,对其种质资源、分类和香气成分进行了研究。然而,其花香形成和调控的分子机制在很大程度上仍然未知。为了从整体上了解开花过程中香气形成的分子机制,我们从三个开花阶段(初花期、盛花期和末花期)构建了 9 个 RNA 测序(RNA-Seq)文库。简而言之,共组装了 136611 条高质量的 unigenes,总共有 523961310 条高质量的 clean reads,平均序列长度为 792bp。约 47.43%(64795 个)的 unigenes可在 NCBI 非冗余蛋白数据库中得到注释。鉴定出萜类代谢途径中的一些候选基因,以及 1327 个转录因子(TFs),它们在花香形成阶段表现出不同的表达模式,特别是、、、和,它们可能在花香形成中发挥关键作用。这些结果表明,瑞香的花香形成是一个非常复杂的过程,涉及大量的 TFs。本研究为进一步研究瑞香的花香形成提供了可靠的资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c2/6100529/81d6e45dbfc8/molecules-23-01604-g001.jpg

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