精炼类固醇生成模型：昆虫前胸腺 RNA-seq 数据集的分析。
Refining a steroidogenic model: an analysis of RNA-seq datasets from insect prothoracic glands.
机构信息
HybridStat Predictive Analytics, Aiolou 19, 10551, Athens, Greece.
Biomedical Sciences Research Center 'Alexander Fleming', Fleming 34, 16672, Vari, Greece.
出版信息
BMC Genomics. 2018 Jul 13;19(1):537. doi: 10.1186/s12864-018-4896-2.
BACKGROUND
The prothoracic gland (PG), the principal steroidogenic organ of insects, has been proposed as a model for steroid hormone biosynthesis and regulation.
RESULTS
To validate the robustness of the model, we present an analysis of accumulated transcriptomic data from PGs of two model species, Drosophila melanogaster and Bombyx mori. We identify that the common core components of the model in both species are encoded by nine genes. Five of these are Halloween genes whose expression differs substantially between the PGs of these species.
CONCLUSIONS
We conclude that the PGs can be a model for steroid hormone synthesis and regulation within the context of mitochondrial cholesterol transport and steroid biosynthesis but beyond these core mechanisms, gene expression in insect PGs is too diverse to fit in a context-specific model and should be analysed within a species-specific framework.
背景
前胸腺（PG）是昆虫主要的类固醇激素合成器官，已被提议作为类固醇激素生物合成和调节的模型。
结果
为了验证该模型的稳健性，我们对两种模式物种果蝇和家蚕的 PG 中的累积转录组数据进行了分析。我们发现，该模型在这两个物种中的共同核心组件由 9 个基因编码。其中 5 个是万圣节基因，它们在这两个物种的 PG 中的表达有很大差异。
结论
我们得出结论，PG 可以作为线粒体胆固醇转运和类固醇生物合成背景下类固醇激素合成和调节的模型，但超出这些核心机制，昆虫 PG 中的基因表达过于多样化，无法适应特定于上下文的模型，应该在特定于物种的框架内进行分析。
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