El-Sayyad Hassan I H, El-Shershaby Effat M F, El-Mansi Ahmed A, El-Ashry Nermeen E
Zoology Dept., Faculty of Science, Mansoura University, Mansoura, Egypt.
Zoology Dept., Faculty of Science, Mansoura University, Mansoura, Egypt; Biology Dept., Faculty of Science, King Khalid University, Abha, Saudi Arabia.
Reprod Biol. 2018 Sep;18(3):236-251. doi: 10.1016/j.repbio.2018.07.003. Epub 2018 Jul 10.
A high cholesterol diet is related to ovarian dysfunction and infertility which has been increased among young ages consuming processed food products. The present study was conducted to evaluate the role of a high cholesterol diet on the ovaries of young female rats via assessments of histopathology, immunohistochemistry, oxidative stress and apoptic markers. Also, mating of hypercholesterolemic female rats was carried out to measure the fertility and numbers of their offspring. At the same time, phytotherapy was carried out through supplementing the diet with barley and/ or date palm fruits (10%) during the experiment to assess the phyto-therapeutic impacts in attenuation of drastic hypercholesterolemic effects. Hypercholesterolemic diet-fed rats exhibited damage of the ovarian follicles and increased follicular atresia. Furthermore, expression of cleaved caspase-3 was upregulated, while PCNA was downregulated in granulosa, theca and stroma cells. Hypercholesterolemic female rats showed marked depletion of antioxidative enzymes, increased lipid peroxidation and apoptotic markers. Alterations to the female serum hormones were detected. Offspring maternally fed on hypercholesterolemic diet showed a significant decrease of body weight and altered sex ratio. However, concomitant supplementation of barley and or date fruits to hypercholesterolemic groups revealed marked improvement of ovarian structure and function. On the basis of these evidences, it is believed that the enhanced synergistic effects of barley and/or date palm fruits in the amelioration of ovarian structure and functions were elicited by the potential antioxidant activity of their phytomicronutrients, polyphenols, β-glucan and trace elements. These materials scavenge free radicals from inflamed cells that can be used to establish an effective and novel therapeutic strategy for activating ovarian cell regeneration.
高胆固醇饮食与卵巢功能障碍和不孕症有关，在食用加工食品的年轻人中，这种情况有所增加。本研究旨在通过组织病理学、免疫组织化学、氧化应激和凋亡标志物评估，来评价高胆固醇饮食对年轻雌性大鼠卵巢的作用。此外，对高胆固醇血症雌性大鼠进行交配，以测量其生育能力和后代数量。同时，在实验期间通过在饮食中添加大麦和/或枣椰果（10%）进行植物疗法，以评估植物疗法对减轻严重高胆固醇血症影响的作用。喂食高胆固醇饮食的大鼠表现出卵巢卵泡损伤和卵泡闭锁增加。此外，颗粒细胞、卵泡膜细胞和基质细胞中裂解的半胱天冬酶-3表达上调，而增殖细胞核抗原表达下调。高胆固醇血症雌性大鼠表现出抗氧化酶明显减少、脂质过氧化和凋亡标志物增加。检测到雌性血清激素发生变化。母源性喂食高胆固醇饮食的后代体重显著下降，性别比例改变。然而，在高胆固醇血症组中同时补充大麦和/或枣果显示卵巢结构和功能有明显改善。基于这些证据，人们认为大麦和/或枣椰果在改善卵巢结构和功能方面增强的协同作用是由其植物微量营养素、多酚、β-葡聚糖和微量元素的潜在抗氧化活性引起的。这些物质清除炎症细胞中的自由基，可用于建立一种有效的新型治疗策略来激活卵巢细胞再生。
