Institute of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, 517502, Andhra Pradesh, India.
Institute of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, 517502, Andhra Pradesh, India.
J Ethnopharmacol. 2019 Jun 28;238:111843. doi: 10.1016/j.jep.2019.111843. Epub 2019 Apr 3.
Hordeum vulgare (L.), commonly known as barley belonging to Poaceae family is a widely used cereal. Barley seeds are considered to possess high nutritional value and antioxidant properties. The grass of barley is also considered as a part of health drink in many parts of India. It is claimed to suppress a number of health disorders including obesity, diabetes, circulatory disorders, arthritis, anemia, excessive cholesterol levels, renal difficulties, and cancer. However, the antiobesity potential of barley grass has not been explored till now.
The aim of the present study was to characterize and evaluate the anti-obesity activity of barley grass juice (Hordeum vulgare L.) in high fat diet induced model.
Barley grass juice was characterized by GC-MS analysis for identifying the active phytochemical constituents and was subjected to standard in vitro antioxidant studies. For in vivo studies, obesity was induced by high fat diet model in adult male Wistar rats. Atorvastatin (10 mg/kg) was used as the standard and barley grass juice was administered at two dose levels (200 and 400 mg/kg) for a period of 60 days. Anthropometric parameters, lipid profile and liver function markers were screened at regular intervals in all the treatment groups. At the end of the study, histopathological evaluations of liver and carotid artery were performed. The levels of in vivo antioxidant enzymes like SOD, catalase, reduced glutathione and lipid peroxidation in terms of malondialdehyde were also estimated in the liver homogenates. Expression levels of PPAR-gamma and caspase 3 were determined in the liver.
Results indicated that barley grass juice (Hordeum vulgare L.) exhibited potent in vitro antioxidant activity. Rats administered with high fat diet for 60 days showed a significant increase in body weight, BMI, altered lipid profile, liver function markers like AST, ALT, ALP and increased expression of PPAR-gamma and caspase 3. However, administration of barley grass juice for 60 days, profoundly decreased the bodyweight, BMI, improved lipid profile and liver function markers. This was supported by the decreased expression of PPAR-gamma and caspase 3 in liver. Histopathological variations observed in liver and carotid artery of high fat diet group, when treated with BJG showed preserved hepatocytes and reduced atherosclerosis. GC-MS analysis identified the presence of 12 phytochemical constituents in barley grass juice.
Our study demonstrates the antiobesity activity of barley grass juice and it may be concluded that barley grass juice can be an effective nutraceutical in the management of obesity.
大麦（L.），通常被称为属于禾本科的大麦，是一种广泛使用的谷物。大麦种子被认为具有很高的营养价值和抗氧化特性。在印度的许多地方，大麦草也被认为是健康饮料的一部分。它被声称可以抑制许多健康问题，包括肥胖、糖尿病、循环障碍、关节炎、贫血、胆固醇水平过高、肾脏困难和癌症。然而，大麦草的减肥潜力至今尚未得到探索。
本研究的目的是对大麦草汁（Hordeum vulgare L.）进行表征，并评估其在高脂肪饮食诱导模型中的抗肥胖活性。
通过 GC-MS 分析对大麦草汁进行了表征，以鉴定其活性植物化学成分，并进行了标准的体外抗氧化研究。在体内研究中，通过高脂肪饮食模型诱导肥胖成年雄性 Wistar 大鼠。阿托伐他汀（10mg/kg）用作标准，大麦草汁以 200 和 400mg/kg 两个剂量水平给药 60 天。在所有治疗组中定期筛查体重、血脂谱和肝功能标志物。在研究结束时，对肝和颈总动脉进行组织病理学评估。还测定了肝匀浆中体内抗氧化酶如 SOD、过氧化氢酶、还原型谷胱甘肽和丙二醛的脂质过氧化水平。在肝中还测定了 PPAR-γ和 caspase 3 的表达水平。
结果表明，大麦草汁（Hordeum vulgare L.）表现出很强的体外抗氧化活性。用高脂肪饮食喂养 60 天的大鼠体重、BMI、血脂谱、肝功能标志物（如 AST、ALT、ALP）显著升高，PPAR-γ和 caspase 3 的表达增加。然而，60 天给予大麦草汁可显著降低体重、BMI、改善血脂谱和肝功能标志物。这得到了肝中 PPAR-γ和 caspase 3 表达降低的支持。用 BJG 处理高脂肪饮食组的肝和颈总动脉的组织病理学变化显示肝细胞保存和动脉粥样硬化减少。GC-MS 分析鉴定出大麦草汁中存在 12 种植物化学成分。
本研究表明大麦草汁具有减肥活性，可以得出结论，大麦草汁可以作为肥胖症管理的有效营养保健品。
