Wang D, Liebowitz D, Kieff E
Cell. 1985 Dec;43(3 Pt 2):831-40. doi: 10.1016/0092-8674(85)90256-9.
Epstein-Barr virus expresses a cytoplasmic and plasma membrane protein (LMP) in latently infected growth transformed lymphocytes. The gene specifying LMP has now been expressed in NIH3T3 and Rat-1 cells. Expression of the gene in these cells resulted in altered cell morphology and some resistance to the growth inhibiting effect of medium containing low serum. In Rat-1 cells, LMP expression often led to loss of contact inhibition and anchorage-independent growth in soft agar. Rat-1 cells expressing LMP were uniformly tumorigenic in nude mice. Thus, LMP is a transforming gene which is likely to account for many aspects of EBV induced cell transformations. This is the first demonstration of a transforming gene in Epstein-Barr virus, a ubiquitous human pathogen associated with neoplasia.
爱泼斯坦-巴尔病毒在潜伏感染的生长转化淋巴细胞中表达一种细胞质和细胞膜蛋白（LMP）。现在，编码LMP的基因已在NIH3T3和大鼠-1细胞中表达。该基因在这些细胞中的表达导致细胞形态改变，并对含低血清培养基的生长抑制作用产生一定抗性。在大鼠-1细胞中，LMP的表达常常导致接触抑制丧失以及在软琼脂中形成非锚定依赖性生长。表达LMP的大鼠-1细胞在裸鼠中均具有致瘤性。因此，LMP是一种转化基因，可能在爱泼斯坦-巴尔病毒诱导的细胞转化的许多方面发挥作用。这是在爱泼斯坦-巴尔病毒（一种与肿瘤形成相关的普遍存在的人类病原体）中首次证明存在转化基因。
