Fairbairn L J, Stewart J P, Hampson I N, Arrand J R, Dexter T M
Cancer Research Campaign Department of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, U.K.
J Gen Virol. 1993 Feb;74 ( Pt 2):247-54. doi: 10.1099/0022-1317-74-2-247.
The product encoded by the latent membrane protein (LMP) gene of Epstein-Barr virus (EBV) has been implicated as a transforming protein by a number of studies. We have examined the effects of LMP expression in FDCP-mix cells, a growth factor-dependent multipotential murine 'stem cell' line. Our studies show that LMP reduces the generation of clonogenic cells and leads to the production of cells expressing a marker (lysozyme M) characteristic of mature monocytes and macrophages. Furthermore, cells expressing LMP are compromised in their ability to produce mature neutrophils. These data suggest that expression of LMP in primitive cells can modulate their self-renewal and differentiation potential and provide evidence in support of the suggestion that EBV may be involved in some of the maturation defects of haemopoiesis.
多项研究表明，爱泼斯坦-巴尔病毒（EBV）的潜伏膜蛋白（LMP）基因编码的产物是一种转化蛋白。我们研究了LMP在FDCP-mix细胞（一种依赖生长因子的多能小鼠“干细胞”系）中的表达效果。我们的研究表明，LMP会减少克隆形成细胞的产生，并导致表达成熟单核细胞和巨噬细胞特征性标志物（溶菌酶M）的细胞生成。此外，表达LMP的细胞产生成熟中性粒细胞的能力受损。这些数据表明，LMP在原始细胞中的表达可调节其自我更新和分化潜能，并为EBV可能参与造血过程中某些成熟缺陷的观点提供了支持证据。
