Vagionitis Stavros, Czopka Tim
Institute of Neuronal Cell Biology, Technical University of Munich, Munich, Germany.
Munich Cluster of Systems Neurology (SyNergy), Munich, Germany.
Methods Mol Biol. 2018;1791:25-35. doi: 10.1007/978-1-4939-7862-5_3.
In vivo time-lapse microscopy provides important information about the kinetics of cellular events and their control by interactions with neighboring cells. Here, we describe the generation and use of transgenic zebrafish to visualize dynamics of myelinating glia using cell type-specific expression and microscopy of genetically encoded fluorescent proteins. With this method, we are able to simultaneously separate and trace up to three different colors over time.
体内延时显微镜技术提供了有关细胞事件动力学及其通过与邻近细胞相互作用进行调控的重要信息。在此，我们描述了转基因斑马鱼的构建及应用，通过细胞类型特异性表达和对基因编码荧光蛋白进行显微镜观察来可视化髓鞘形成胶质细胞的动态变化。利用这种方法，我们能够随时间同时分离并追踪多达三种不同颜色。
