Department of Histology and Embryology, Medical Academy, Lithuanian University of Health Sciences, A. Mickeviciaus str. 9, LT-44307, Kaunas, Lithuania.
Kaunas Division of State Forensic Medicine Service, Perlojos str. 28, LT-45305, Kaunas, Lithuania.
Reprod Biol Endocrinol. 2018 Jul 14;16(1):66. doi: 10.1186/s12958-018-0384-4.
For the first time, thorough morphometrical measurements of primordial ovarian follicles were performed and their age-related changes were investigated in Lithuanian women of the reproductive age.
Ovaries of dead women (n = 30) were divided into six age groups: 15-20 years old, 21-25 years old, 26-30 years old, 31-35 years old, 36-40 years old and 41-46 years old. Histological slides of left and right ovaries were stained using haematoxylin-eosin and periodic acid-Schiff (PAS) staining methods. The morphometrical measurements of 10 primordial ovarian follicles of the left and right ovary of each woman were made from microphotographs.
The diameter of primordial ovarian follicles increased in groups of women from 15 years old to 35 years old and decreased in the groups from 36 years old to 46 years old. The area of primordial ovarian follicles increased in the groups of women until 35 years old. It decreased in the groups of women older than 36 years. The follicular basement membrane thickened from 1.29 ± 0.11 μm to 1.43 ± 0.18 μm with increasing age of women. The diameter of primary oocytes enlarged until 35 years and then began to decrease. The area of primary oocytes increased in women until 35 years. It decreased in groups of women aged 36-40 and 41-46 years old. The diameter and the area of primary oocytes nuclei increased in women aged 15-30 years old; later, it began to decrease. The length of follicular cells varied from 8.56 ± 0.43 μm to 8.72 ± 0.27 μm (p > 0.05). The height of follicular cells varied from 2.59 ± 0.27 μm to 2.7 ± 0.21 μm (p > 0.05). The diameter, the area and the basement membrane thickness of primordial ovarian follicles and the diameter and the area of primary oocytes and their nuclei differed insignificantly in left and right ovaries in all age groups of women (p > 0.5). The length and height of follicular cells were similar in left and right ovaries of the same age group (p > 0.5).
The age decreasing of morphometrical parameters begins in primordial ovarian follicles and their primary oocytes in Lithuanian women older than 35 years. The thickness of the follicular basement membrane increased with increasing age of women. No significant differences were found in the morphometrical parameters in primordial follicles of left and right ovaries in the same age group of women.
本研究首次对立陶宛育龄期女性的原始卵泡进行了全面的形态测量，并研究了其与年龄相关的变化。
对 30 名死亡女性的卵巢进行了分组，分为六个年龄组：15-20 岁、21-25 岁、26-30 岁、31-35 岁、36-40 岁和 41-46 岁。使用苏木精-伊红和过碘酸希夫（PAS）染色方法对左、右卵巢的组织切片进行染色。从每位女性的左、右卵巢的 10 个原始卵泡的显微照片中进行形态测量。
原始卵泡的直径在 15 岁至 35 岁的女性组中增加，在 36 岁至 46 岁的女性组中减少。原始卵泡的面积在 35 岁前的女性组中增加。在 36 岁以上的女性组中减少。卵泡基底膜厚度从 1.29±0.11μm增加到 1.43±0.18μm，随着女性年龄的增长而增加。初级卵母细胞的直径在 35 岁前增大，然后开始减小。初级卵母细胞的面积在 35 岁前的女性组中增加。在 36-40 岁和 41-46 岁的女性组中减少。15-30 岁的女性中原始卵泡核的直径和面积增加；之后开始减少。卵泡细胞的长度在 8.56±0.43μm至 8.72±0.27μm之间变化（p>0.05）。卵泡细胞的高度在 2.59±0.27μm至 2.7±0.21μm之间变化（p>0.05）。在所有年龄组的女性中，左右卵巢的原始卵泡和初级卵母细胞及其核的形态学参数直径、面积和基底膜厚度差异均无统计学意义（p>0.5）。同一年龄组左右卵巢的卵泡细胞长度和高度相似（p>0.5）。
立陶宛 35 岁以上女性的原始卵泡及其初级卵母细胞的形态学参数随年龄的增长而减少。随着女性年龄的增长，卵泡基底膜的厚度增加。同一年龄组女性左右卵巢原始卵泡的形态学参数无显著差异。
