Department of Analytical Chemistry, School of Sciences, China Pharmaceutical University, Nanjing 210009, Jiangsu, China.
Department of Analytical Chemistry, School of Sciences, China Pharmaceutical University, Nanjing 210009, Jiangsu, China.
Carbohydr Polym. 2018 Oct 1;197:117-127. doi: 10.1016/j.carbpol.2018.05.071. Epub 2018 May 28.
In this research, to achieve enhanced intracellular uptake of anticancer drug carriers for efficient chemotherapy, folic acid conjugated chitosan oligosaccharides assembled magnetic halloysite nanotubes (FA-COS/MHNTs) have been tailored as multitask drug delivery system towards camptothecin (CPT). Besides magnetic targeting, the nanocomposites have been reacted with folate complex in order to selectively target cancer cells over expressing the folic acid receptor. HNTs showed to have a high storage capacity of CPT. In vitro, the release results indicated that CPT outflow from the nanocarriers at pH 5 was much greater than that at both pH 6.8 and 7.4. MTT assays showed that the CPT-loaded nanocarriers exhibited stronger cell growth inhibitory against colon cancer cell. Furthermore, nanocarriers gained specificity to target cancer cells because of the enhanced cell uptake mediated by FA moiety and presence of COS. Therefore, the rational designed HNTs nanocarrier for chemotherapy drug showed great potential as tumor-targeted drug delivery carrier.
在这项研究中，为了实现抗癌药物载体的增强细胞内摄取，以实现有效的化学疗法，将叶酸偶联壳聚糖寡糖组装的磁性埃洛石纳米管（FA-COS/MHNTs）作为多功能药物输送系统，针对喜树碱（CPT）。除了磁靶向外，纳米复合材料还与叶酸复合物反应，以便选择性地针对过度表达叶酸受体的癌细胞。HNTs 显示出对 CPT 的高储存能力。在体外，释放结果表明，CPT 从纳米载体在 pH5 下的流出量远大于在 pH6.8 和 7.4 下的流出量。MTT 测定表明，载有 CPT 的纳米载体对结肠癌细胞表现出更强的细胞生长抑制作用。此外，由于 FA 部分和 COS 的存在介导的细胞摄取增强，纳米载体获得了针对癌细胞的特异性。因此，合理设计的用于化疗药物的 HNTs 纳米载体作为肿瘤靶向药物输送载体具有很大的潜力。
