金针菇新型多糖的结构特征和生物活性性质。
Structural characteristics and bioactive properties of a novel polysaccharide from Flammulina velutipes.
机构信息
College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China.
Department of Nutrition and Food Hygiene, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, Guangdong, China.
出版信息
Carbohydr Polym. 2018 Oct 1;197:147-156. doi: 10.1016/j.carbpol.2018.05.069. Epub 2018 May 25.
A new water-soluble polysaccharide (FVP1) was extracted from Flammulina velutipes by traditional method "water extraction and alcohol precipitation" and purified by column chromatography. Physicochemical characterization showed that FVP1 was a homogeneous polysaccharide with a relative molecular weight of 54.78 kDa. It is composed of mannose (7.74%), glucose (70.41%), and galactose (16.38%). FVP1 (1000 mg/mL) possessed significant immune activity by increasing the secretion of nitric oxide (NO), tumour necrosis factor-α (TNF-α) (3183 ± 133.84 pg/mL), interleukin (IL)-6 (1133.21 ± 39.05 pg/mL), and IL-12 (579.96 ± 74.53 pg/mL) in macrophages. Furthermore, FVP1 showed significant hepatitis B surface antibody (anti-HBV) activity through reducing the expression of hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg) and hepatitis B virus (HBV) DNA replication. These results suggest a novel role for FVP1 to be applied as an immunomodulators in dietary supplements to prevent HBV infection.
一种新的水溶性多糖（FVP1）从金针菇通过传统方法“水提取和醇沉淀”提取和纯化通过柱层析。理化特性表明 FVP1 是一种均相多糖，相对分子量为 54.78 kDa。它由甘露糖（7.74%）、葡萄糖（70.41%）和半乳糖（16.38%）组成。FVP1（1000 mg/mL）通过增加一氧化氮（NO）、肿瘤坏死因子-α（TNF-α）（3183±133.84 pg/mL）、白细胞介素（IL）-6（1133.21±39.05 pg/mL）和白细胞介素（IL）-12（579.96±74.53 pg/mL）的分泌而具有显著的免疫活性。此外，FVP1 通过降低乙型肝炎表面抗原（HBsAg）、乙型肝炎 e 抗原（HBeAg）和乙型肝炎病毒（HBV）DNA 复制的表达，显示出显著的乙型肝炎表面抗体（抗 HBV）活性。这些结果表明，FVP1 可作为一种免疫调节剂应用于膳食补充剂中，以预防 HBV 感染。
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