亚麻籽杂多糖可刺激免疫应答并抑制乙型肝炎病毒。
A flaxseed heteropolysaccharide stimulates immune responses and inhibits hepatitis B virus.
机构信息
Guangdong Saskatchewan Oilseed Joint Laboratory, Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China; Guangdong Engineering Technology Research Center for Oils and Fats Biorefinery, Guangzhou 510632, China; Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Guangdong Saskatchewan Oilseed Joint Laboratory, Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China; Guangdong Engineering Technology Research Center for Oils and Fats Biorefinery, Guangzhou 510632, China; Guangzhou Institute of Microbiology, Guangzhou 510663, China.
出版信息
Int J Biol Macromol. 2019 Sep 1;136:230-240. doi: 10.1016/j.ijbiomac.2019.06.076. Epub 2019 Jun 12.
A novel heteropolysaccharide defined as FP-1 was enriched from flaxseed hull through hot water extraction (boiling water, 2 h, twice) followed by ion-exchange and size exclusion chromatography. FP-1 is a highly purified heteropolysaccharide with an average molecular weight of 2626 kDa. Its monosaccharide composition includes xylose (35.2%), rhamnose (23.8%), galactose (23.4%), arabinose (10.6%), fucose (4.0%), glucose (2.7%) and mannose (0.3%). Main linkage type of FP-1 backbone includes →2)-α-L-Rhap-(1→, →4)-α-D-GalpA-(1→, →4)-β-D-Xylp-(1→, →3,5)-α-L-Araf-(1→, and →2)-α-D-Xylp-(1→. FP-1 has a triple-helix conformation, indicating its potential bioactivity. FP-1 could stimulate immune responses through inducing mRNA expression of tumor necrosis factor α (TNF-α), nitric oxide (NO), and interleukin (IL-6 and IL-12) in murine macrophages. FP-1 also inhibits hepatitis B virus (HBV) through inhibition of surface antigen (HBsAg) and envelope antigen (HBeAg) expression and interfering with HBV DNA replication. These findings suggest that FP-1 could be potentially developed as functional food ingredient, immune stimulant and vaccine adjuvant.
一种新型杂多糖 FP-1 是从亚麻籽壳中通过热水提取（沸水，2 小时，2 次），然后进行离子交换和凝胶渗透色谱分离得到的。FP-1 是一种高度纯化的杂多糖，平均分子量为 2626 kDa。其单糖组成包括木糖（35.2%）、鼠李糖（23.8%）、半乳糖（23.4%）、阿拉伯糖（10.6%）、岩藻糖（4.0%）、葡萄糖（2.7%）和甘露糖（0.3%）。FP-1 主链的主要连接类型包括→2)-α-L-Rhap-(1→、→4)-α-D-GalpA-(1→、→4)-β-D-Xylp-(1→、→3,5)-α-L-Araf-(1→和→2)-α-D-Xylp-(1→。FP-1 具有三螺旋构象，表明其具有潜在的生物活性。FP-1 可通过诱导鼠巨噬细胞肿瘤坏死因子 α（TNF-α）、一氧化氮（NO）和白细胞介素（IL-6 和 IL-12）的 mRNA 表达来刺激免疫反应。FP-1 还通过抑制表面抗原（HBsAg）和包膜抗原（HBeAg）的表达以及干扰 HBV DNA 复制来抑制乙型肝炎病毒（HBV）。这些发现表明，FP-1 可能被开发为功能性食品成分、免疫刺激剂和疫苗佐剂。
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