Food Engineering and Packaging Department, Food Technology Research Institute, Agricultural Research Center, Giza, Egypt.
Center for Humanities and Sciences, Bionanocomposite Research Center, Department of Food and Nutrition, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.
Carbohydr Polym. 2018 Oct 1;197:349-358. doi: 10.1016/j.carbpol.2018.06.033. Epub 2018 Jun 14.
Chitin nanocrystal (ChNC) was isolated using sulfuric acid hydrolysis (ChNC), TEMPO-oxidation (ChNC), and ammonium persulfate (ChNC) methods, and used for the preparation of hybrid nanoparticles of ChNC/silver nanoparticles (AgNP). The ChNC exhibited a needle-shaped structure with a sulfate group content of 135 μmol/g for ChNC and carboxyl content of 0.71 and 1.42 mmol/g for ChNC and ChNC, respectively. ChNC worked as a reducing and stabilizing agent for the production of AgNP and reduced the size of AgNP from 23.9 nm to 6.3 nm in the ChNC/AgNP hybrid. The carboxyl content of ChNC played a significant role for the nucleation, size distribution, and antibacterial activity of ChNC/AgNP. ChNC/AgNP hybrid, especially ChNC/AgNP, exhibited strong antibacterial activity against food-borne pathogenic Gram-negative (E. coli) and Gram-positive (L. monocytogenes) bacteria. The prepared ChNC/AgNP hybrid nanomaterials have a high potential for the application to be used as a nanofiller to improve the properties of food packaging materials to extend the shelf-life of packaged food.
壳聚糖纳米晶（ChNC）分别采用硫酸水解（ChNC）、TEMPO 氧化（ChNC）和过硫酸铵（ChNC）方法进行分离，并用于制备壳聚糖纳米晶/银纳米颗粒（AgNP）的杂化纳米颗粒。ChNC 呈针状结构，硫酸根含量为 135 μmol/g，ChNC 和 ChNC 的羧基含量分别为 0.71 和 1.42 mmol/g。ChNC 作为生产 AgNP 的还原剂和稳定剂，将 AgNP 的尺寸从 ChNC/AgNP 杂化中的 23.9nm 减小到 6.3nm。ChNC 的羧基含量对 ChNC/AgNP 的成核、尺寸分布和抗菌活性起着重要作用。ChNC/AgNP 杂化，特别是 ChNC/AgNP，对食源性病原体革兰氏阴性（大肠杆菌）和革兰氏阳性（单核细胞增生李斯特菌）具有很强的抗菌活性。所制备的 ChNC/AgNP 杂化纳米材料具有作为纳米填充剂应用的巨大潜力，可改善食品包装材料的性能，延长包装食品的保质期。
