College of Plant Protection, Southwest University, Chongqing, China.
Chongqing Key Laboratory of Soft-Matter Material Chemistry and Function Manufacturing, Southwest University, Chongqing, China.
Pest Manag Sci. 2024 Mar;80(3):1076-1086. doi: 10.1002/ps.7838. Epub 2023 Nov 6.
In recent years, nanomaterials-based pesticide carriers have garnered significant attention and sparked extensive research. However, most studies have primarily focused on investigating the impact of physical properties of nanomaterials, such as size and modifiable sites, on drug delivery efficiency of nano-pesticides. The limited exploration of biologically active nanomaterials poses a significant obstacle to the advancement and widespread adoption of nano-pesticides. In this study, we prepared chitin nanocrystals (ChNC) based on acid hydrolysis and systematically investigated the differences between nano- and normal chitin against plant bacteria (Pseudomonas syringae pv. tabaci). The primary objective was to seek out nanocarriers with heightened biological activity for the synthesis of nano-pesticides.
Zeta potential analysis, Fourier Transform infrared spectrometry (FTIR), X-Ray diffraction (XRD), Atomic force microscopy (AFM) and Transmission electron microscopy (TEM) identified the successful synthesis of ChNC. ChNC showcased remarkable bactericidal activity at comparable concentrations, surpassing that of chitin, particularly in its ability to inhibit bacterial biofilm formation. Furthermore, ChNC displayed heightened effectiveness in disrupting bacterial cell membranes, resulting in the leakage of bacterial cell contents, structural DNA damage, and impairment of DNA replication. Lastly, potting experiments revealed that ChNC is notably more effective in inhibiting the spread and propagation of bacteria on plant leaves.
ChNC exhibited higher antibacterial activity compared to chitin, enabling efficient control of plant bacterial diseases through enhanced interaction with bacteria. These findings offer compelling evidence of ChNC's superior bacterial inhibition capabilities, underscoring its potential as a promising nanocarrier for nano-pesticide research. © 2023 Society of Chemical Industry.
近年来，基于纳米材料的农药载体受到了广泛关注，并引发了大量研究。然而，大多数研究主要集中在研究纳米材料的物理性质，如大小和可修饰位点，对纳米农药的药物输送效率的影响。对具有生物活性的纳米材料的有限探索对纳米农药的发展和广泛采用构成了重大障碍。在本研究中，我们基于酸水解制备了壳聚糖纳米晶体（ChNC），并系统地研究了纳米和普通壳聚糖对植物细菌（丁香假单胞菌 pv. tabaci）的差异。主要目的是寻找具有更高生物活性的纳米载体来合成纳米农药。
Zeta 电位分析、傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）、原子力显微镜（AFM）和透射电子显微镜（TEM）鉴定了 ChNC 的成功合成。在可比浓度下，ChNC 表现出显著的杀菌活性，优于壳聚糖，特别是在抑制细菌生物膜形成方面。此外，ChNC 在破坏细菌细胞膜方面表现出更高的效果，导致细菌细胞内容物泄漏、结构 DNA 损伤和 DNA 复制受损。最后，盆栽实验表明，ChNC 在抑制细菌在植物叶片上的传播和繁殖方面明显更有效。
ChNC 表现出比壳聚糖更高的抗菌活性，通过与细菌的有效相互作用，能够有效控制植物细菌病害。这些发现有力地证明了 ChNC 具有优越的抑菌能力，突显了其作为纳米农药研究有前途的纳米载体的潜力。 © 2023 化学工业协会。
