Yang Xian-Zi, He Qing-Jun, Cheng Tian-Tian, Chi Jun, Lei Zi-Ying, Tang Zhen, Liao Quan-Xing, Zhang Hong, Zeng Li-Si, Cui Shu-Zhong
Department of Medical Oncology, Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, China.
Department of Abdominal Surgery, Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, China.
Cell Physiol Biochem. 2018;48(1):251-262. doi: 10.1159/000491724. Epub 2018 Jul 13.
BACKGROUND/AIMS: Considerable evidence indicates that long noncoding RNAs (lncRNAs) exert importantly regulatory functions during human cancer initiation and progression and are promising biotargets in the flight against cancer.
In this study, we evaluated the role of the lncRNA LINC01133 in esophageal squamous cell carcinoma (ESCC). LINC01133 expression in ESCC was examined by quantitative real-time PCR. The correlations between LINC01133 expression and clinicopathological variables and survival were examined by the χ2 test, Kaplan-Meier method, log-rank test, and univariate Cox regression analysis.
LINC01133 expression levels were frequently lower in ESCC tissues and cell lines than in paired normal tissues and an immortalized esophageal epithelial cell line, respectively. The expression of LINC01133 decreased in a TNM stage- and lifestyle-independent manner. LINC01133 was an independent protective factor and had an anti-tumor effect in the early stage of ESCC development. More importantly, we discovered that drinking status in our cohort impaired the predictive accuracy of LINC01133 for patients with ESCC. Furthermore, a new risk model combining LINC01133 expression, drinking status, and TNM stage provided better survival discrimination compared with three other predictors.
Our data indicate that a loss of LINC01133 expression is a potential poor prognostic biomarker and therapeutic target for ESCC and provide additional prognostic information to improve the outcomes of ESCC patients.
背景/目的：大量证据表明，长链非编码RNA（lncRNA）在人类癌症的发生和发展过程中发挥着重要的调节功能，是抗癌斗争中很有前景的生物靶点。
在本研究中，我们评估了lncRNA LINC01133在食管鳞状细胞癌（ESCC）中的作用。通过定量实时PCR检测ESCC中LINC01133的表达。采用χ2检验、Kaplan-Meier法、对数秩检验和单因素Cox回归分析来检测LINC01133表达与临床病理变量及生存率之间的相关性。
ESCC组织和细胞系中LINC01133的表达水平分别经常低于配对的正常组织和永生化食管上皮细胞系。LINC01133的表达以与TNM分期和生活方式无关的方式降低。LINC01133是一个独立的保护因素，在ESCC发展的早期具有抗肿瘤作用。更重要的是，我们发现我们队列中的饮酒状况会损害LINC01133对ESCC患者的预测准确性。此外，与其他三个预测指标相比，一个结合了LINC01133表达、饮酒状况和TNM分期的新风险模型能提供更好的生存区分度。
我们的数据表明，LINC01133表达缺失是ESCC潜在的不良预后生物标志物和治疗靶点，并提供了额外的预后信息以改善ESCC患者的治疗结果。
