Amstalden Maria-Cecília Krähenbühl, Oliveira José Diogo, Strauss Mathias, Mazali Italo Odone, Machado Daisy, Theisen Thais Holtz, Lancellotti Marcelo
Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, SP, Brazil.
Faculty of Pharmaceutical Sciences, University of Campinas, Campinas, SP, Brazil.
Folia Microbiol (Praha). 2019 Jan;64(1):127-132. doi: 10.1007/s12223-018-0631-2. Epub 2018 Jul 14.
Streptococcus pneumoniae are natural competent bacteria which requires the presence of a pheromone-like molecule to do the transformation process. This study verified the influence of mesoporous silica (SBA-15 and SBA-16) on the transformation process in S. pneumoniae using a donor DNA obtained from a mutant strain of this microorganism (Sp360∆luxS). The results showed that mesoporous silica SBA-15 and SBA-16 particles doubled the transformation ratio frequency compared with negative control (without nanoparticles) in using SBA-15 (ratio 1.81 ± 0.04) and SBA-16 (ratio 2.18 ± 0.22). We demonstrated the how mesoporous silica nanoparticles were able to increase the pneumococcus transformations, which could possibly lead to the acquisition of virulence factor genes and resistance of antibiotics.
肺炎链球菌是天然感受态细菌，其转化过程需要一种类似信息素的分子存在。本研究使用从该微生物的突变菌株（Sp360∆luxS）获得的供体DNA，验证了介孔二氧化硅（SBA - 15和SBA - 16）对肺炎链球菌转化过程的影响。结果表明，与阴性对照（无纳米颗粒）相比，介孔二氧化硅SBA - 15和SBA - 16颗粒使转化比率频率提高了一倍，使用SBA - 15时（比率为1.81±0.04），使用SBA - 16时（比率为2.18±0.22）。我们证明了介孔二氧化硅纳米颗粒如何能够增加肺炎球菌的转化，这可能导致毒力因子基因的获得和抗生素耐药性。
