Microbiology Program in Science, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand.
Mar Pollut Bull. 2013 Sep 15;74(1):95-104. doi: 10.1016/j.marpolbul.2013.07.025. Epub 2013 Aug 5.
PAH-degrading bacteria, including Novosphingobium sp. PCY, Microbacterium sp. BPW, Ralstonia sp. BPH, Alcaligenes sp. SSK1B, and Achromobacter sp. SSK4, were isolated from mangrove sediments. These isolates degraded 50-76% of 100 mg/l phenanthrene within 2 weeks. Strains PCY and BPW also degraded pyrene at 98% and 71%, respectively. Furthermore, all of them probably produced biosurfactants in the presence of hydrocarbons. Interestingly, PCY has a versatility to degrade various PAHs. Molecular techniques and plasmid curing remarkably revealed the presence of the alpha subunit of pyrene dioxygenase gene (nidA), involving in its pyrene/phenanthrene degrading ability, located on megaplasmid of PCY which has never before been reported in sphingomonads. Moreover, genes encoding ferredoxin, reductase, extradiol dioxygenase (bphA3A4C) and exopolysaccharide biosynthetase, which may be involved in PAH degradation and biosurfactant production, were also found in PCY. Therefore, we conclude that these isolates, especially PCY, can be the candidates for use as inoculums in the bioremediation.
从红树林沉积物中分离出了降解多环芳烃（PAH）的细菌，包括新鞘氨醇单胞菌（Novosphingobium sp.）PCY、微杆菌（Microbacterium sp.）BPW、罗尔斯顿氏菌（Ralstonia sp.）BPH、产碱杆菌（Alcaligenes sp.）SSK1B 和无色杆菌（Achromobacter sp.）SSK4。这些分离株在 2 周内降解了 100mg/L 菲的 50-76%。PCY 和 BPW 菌株也分别降解了 98%和 71%的芘。此外，它们可能都在烃类存在的情况下产生了生物表面活性剂。有趣的是，PCY 具有降解各种多环芳烃的多功能性。分子技术和质粒消除显著揭示了参与其芘/菲降解能力的芘双加氧酶基因（nidA）的α亚基的存在，该基因位于 PCY 的巨大质粒上，这在鞘氨醇单胞菌中从未报道过。此外，还发现了编码铁氧还蛋白、还原酶、外二醇加氧酶（bphA3A4C）和胞外多糖生物合成酶的基因，这些基因可能参与多环芳烃的降解和生物表面活性剂的产生。因此，我们得出结论，这些分离株，尤其是 PCY，可以作为生物修复中的接种物候选。
