猪囊尾蚴病：酶联免疫电转印印迹法中针对GP50抗原的可能交叉反应性。
Porcine Cysticercosis: Possible Cross-Reactivity of to GP50 Antigen in the Enzyme-Linked Immunoelectrotransfer Blot Assay.
作者信息
Muro Claudio, Gomez-Puerta Luis A, Flecker Robert H, Gamboa Ricardo, Barreto Percy Vilchez, Dorny Pierre, Tsang Victor C W, Gilman Robert H, Gonzalez Armando E, Garcia Hector H, O'Neal Seth E
机构信息
Center for Global Health Tumbes, Universidad Peruana Cayetano Heredia, Tumbes, Peru.
School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru.
出版信息
Am J Trop Med Hyg. 2017 Dec;97(6):1830-1832. doi: 10.4269/ajtmh.17-0378. Epub 2017 Sep 28.
The lentil lectin glycoprotein enzyme-linked immunoelectrotransfer blot (LLGP EITB, reported sensitivity 99% and specificity 100%) is used as a serologic marker of exposure to in pigs. However, only a limited number of parasites have been evaluated for cross reactivity. Pigs may host other related cestode infections, including which have not been formally evaluated for cross-reactions. We investigated a corral in Tumbes, Peru, a region where a cysticercosis elimination demonstration project was completed in 2012. In this corral, 14/19 (73.7%) 6-8-week-old piglets were reactive to GP50 on LLGP EITB, and all had circulating sp. antigens. From eight necropsied piglets; four were infected with metacestodes whereas none had evidence of infection. Two resident dogs were subsequently confirmed to have taeniasis. These results suggest GP50 cross-reactivity in infected pigs, although controlled experimental infection is needed to confirm this hypothesis.
扁豆凝集素糖蛋白酶联免疫电转移印迹法（LLGP EITB，报道的敏感性为99%，特异性为100%）被用作猪感染[寄生虫名称未给出]的血清学标志物。然而，仅对有限数量的寄生虫进行了交叉反应性评估。猪可能感染其他相关的绦虫，包括[未给出的绦虫名称]，尚未对其交叉反应进行正式评估。我们调查了秘鲁通贝斯的一个畜栏，该地区在2012年完成了一项囊尾蚴病消除示范项目。在这个畜栏中，14/19（73.7%）的6 - 8周龄仔猪在LLGP EITB上对GP50呈反应性，并且所有仔猪都有循环的[寄生虫名称未给出]抗原。对八只剖检的仔猪进行检查，四只感染了[绦虫名称未给出]的中绦期幼虫，而没有一只显示出[寄生虫名称未给出]感染的迹象。随后证实两只常住犬患有[绦虫名称未给出]绦虫病。这些结果表明感染猪中存在GP50交叉反应性，尽管需要进行对照实验感染来证实这一假设。
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