编码人肝脏乙醇脱氢酶β亚基的cDNA克隆具有不同大小的3'非编码区。

cDNA clones coding for the beta-subunit of human liver alcohol dehydrogenase have differently sized 3'-non-coding regions.

作者信息

Hedén L O, Höög J O, Larsson K, Lake M, Lagerholm E, Holmgren A, Vallee B L, Jörnvall H, von Bahr-Lindström H

出版信息

FEBS Lett. 1986 Jan 6;194(2):327-32. doi: 10.1016/0014-5793(86)80111-9.

Abstract

Three different size classes of cDNA clones coding for the beta 1-subunit of human alcohol dehydrogenase (ADH) were characterized from a human liver cDNA library. Clones were identified by hybridization with synthetic oligodeoxyribonucleotides. A total of 2530 nucleotides were determined, covering an ADH-coding region of 1122 nucleotides, a preceding 72-nucleotide segment and 3 types of 3'-non-coding region. The coding nucleotide sequence is in full agreement with the amino acid sequence of the beta 1-subunit. Of 8 clones identified, 6 had a short, 213-nucleotide 3'-non-coding region; 1 an intermediate, 590-nucleotide 3'-region; and 1 a long, 1330-nucleotide 3'-region. In addition, 2 unused polyadenylation signals were found. These results suggest that human liver beta-ADH mRNAs occur in several size classes, and that in addition to the consensus sequence AATAAA further signals are important for 3'-end formation.

摘要

从人肝脏cDNA文库中鉴定出编码人乙醇脱氢酶（ADH）β1亚基的三种不同大小类别的cDNA克隆。通过与合成寡脱氧核糖核苷酸杂交来鉴定克隆。共测定了2530个核苷酸，涵盖1122个核苷酸的ADH编码区、前面72个核苷酸的片段以及3种3'非编码区。编码核苷酸序列与β1亚基的氨基酸序列完全一致。在鉴定出的8个克隆中，6个具有短的213个核苷酸的3'非编码区；1个具有中等长度的590个核苷酸的3'区；1个具有长的1330个核苷酸的3'区。此外，还发现了2个未使用的聚腺苷酸化信号。这些结果表明，人肝脏β-ADH mRNA存在几种大小类别，并且除了共有序列AATAAA之外，其他信号对3'末端形成也很重要。