人乙醇脱氢酶β亚基cDNA的分子克隆与特性分析
Molecular cloning and characterization of a cDNA for the beta subunit of human alcohol dehydrogenase.
作者信息
Duester G, Hatfield G W, Bühler R, Hempel J, Jörnvall H, Smith M
出版信息
Proc Natl Acad Sci U S A. 1984 Jul;81(13):4055-9. doi: 10.1073/pnas.81.13.4055.
Human alcohol dehydrogenase (ADH) is encoded by at least five genes that fall into three classes. The class I ADH genes encode the three closely related alpha, beta, and gamma polypeptides. Molecular genetic analysis of class I ADH genes has been initiated by isolating a cDNA clone from a human adult liver cDNA library. A synthetic oligonucleotide mixture encoding a portion of the beta subunit of ADH was used as an in situ hybridization probe for the cDNA library. One positively hybridizing clone, pADH12, which contained an 1100-base-pair cDNA insert, was subjected to DNA sequence analysis. The sequence indicated that the cDNA encoded information for the carboxyl-terminal 91 amino acids of a class I ADH and a 3' untranslated region of 593 nucleotides. Comparisons with the carboxyl terminus of the human ADH beta subunit indicated that the cDNA encoded the beta polypeptide. This probe may facilitate genetic studies of various human alcohol-related syndromes, as well as enable basic molecular studies on human ADH gene expression.
人类乙醇脱氢酶（ADH）由至少五个基因编码，这些基因分为三类。I类ADH基因编码三种密切相关的α、β和γ多肽。通过从人类成人肝脏cDNA文库中分离出一个cDNA克隆，开始了对I类ADH基因的分子遗传学分析。一种编码ADHβ亚基一部分的合成寡核苷酸混合物被用作cDNA文库的原位杂交探针。一个阳性杂交克隆pADH12，其包含一个1100个碱基对的cDNA插入片段，进行了DNA序列分析。序列表明，该cDNA编码I类ADH羧基末端91个氨基酸的信息以及一个593个核苷酸的3'非翻译区。与人类ADHβ亚基的羧基末端比较表明，该cDNA编码β多肽。该探针可能有助于对各种人类酒精相关综合征进行遗传学研究，也能够对人类ADH基因表达进行基础分子研究。
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