• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Cloning and sequencing of cDNA encoding the complete mouse liver alcohol dehydrogenase.编码完整小鼠肝脏乙醇脱氢酶的cDNA的克隆与测序
Proc Natl Acad Sci U S A. 1985 Apr;82(8):2262-6. doi: 10.1073/pnas.82.8.2262.
2
Cloning and sequencing of cDNA encoding baboon liver alcohol dehydrogenase: evidence for a common ancestral lineage with the human alcohol dehydrogenase beta subunit and for class I ADH gene duplications predating primate radiation.狒狒肝脏乙醇脱氢酶编码cDNA的克隆与测序：与人类乙醇脱氢酶β亚基拥有共同祖先谱系及灵长类辐射前I类乙醇脱氢酶基因重复的证据
Proc Natl Acad Sci U S A. 1989 Jul;86(14):5454-8. doi: 10.1073/pnas.86.14.5454.
3
Molecular cloning and characterization of a cDNA for the beta subunit of human alcohol dehydrogenase.人乙醇脱氢酶β亚基cDNA的分子克隆与特性分析
Proc Natl Acad Sci U S A. 1984 Jul;81(13):4055-9. doi: 10.1073/pnas.81.13.4055.
4
Molecular cloning of mouse alcohol dehydrogenase-B2 cDNA: nucleotide sequences of the class III ADH genes evolve slowly even for silent substitutions.小鼠乙醇脱氢酶-B2 cDNA 的分子克隆：即使对于沉默替代，III 类乙醇脱氢酶基因的核苷酸序列进化也很缓慢。
DNA Seq. 1992;3(3):167-75. doi: 10.3109/10425179209034012.
5
Three human alcohol dehydrogenase subunits: cDNA structure and molecular and evolutionary divergence.三种人类乙醇脱氢酶亚基：cDNA结构、分子及进化差异
Proc Natl Acad Sci U S A. 1986 Feb;83(3):634-8. doi: 10.1073/pnas.83.3.634.
6
Androgen induction of alcohol dehydrogenase in mouse kidney. Studies with a cDNA probe confirmed by nucleotide sequence analysis.雄激素对小鼠肾脏中乙醇脱氢酶的诱导作用。通过核苷酸序列分析证实的cDNA探针研究。
Gene. 1986;41(2-3):217-24. doi: 10.1016/0378-1119(86)90101-0.
7
Complete amino acid sequence of rat liver alcohol dehydrogenase deduced from the cDNA sequence.从cDNA序列推导的大鼠肝脏乙醇脱氢酶的完整氨基酸序列。
Gene. 1986;48(2-3):287-91. doi: 10.1016/0378-1119(86)90087-9.
8
Molecular cloning of a full-length cDNA for human alcohol dehydrogenase.人酒精脱氢酶全长cDNA的分子克隆
Proc Natl Acad Sci U S A. 1985 May;82(9):2703-7. doi: 10.1073/pnas.82.9.2703.
9
Cloning and comparative mapping of a human class III (chi) alcohol dehydrogenase cDNA.人类Ⅲ类（χ）乙醇脱氢酶cDNA的克隆与比较定位
Biochem Biophys Res Commun. 1989 Oct 16;164(1):453-60. doi: 10.1016/0006-291x(89)91741-5.
10
Alcohol dehydrogenase gene of Drosophila melanogaster: relationship of intervening sequences to functional domains in the protein.黑腹果蝇的乙醇脱氢酶基因：间隔序列与蛋白质功能结构域的关系。
Proc Natl Acad Sci U S A. 1981 May;78(5):2717-21. doi: 10.1073/pnas.78.5.2717.

引用本文的文献

1
Cloning and Expression of a Clostridium acetobutylicum Alcohol Dehydrogenase Gene in Escherichia coli.丙酮丁醇梭菌乙醇脱氢酶基因在大肠杆菌中的克隆与表达
Appl Environ Microbiol. 1988 Mar;54(3):676-682. doi: 10.1128/aem.54.3.676-682.1988.
2
Experimental Assessment of the Role of Acetaldehyde in Alcoholic Cardiomyopathy.乙醛在酒精性心肌病中作用的实验评估
Biol Proced Online. 2003;5:1-12. doi: 10.1251/bpo41. Epub 2003 Feb 17.
3
Liver-enriched transcription factors uncoupled from expression of hepatic functions in hepatoma cell lines.肝癌细胞系中肝脏富集转录因子与肝脏功能表达解偶联。
Mol Cell Biol. 1997 Nov;17(11):6311-20. doi: 10.1128/MCB.17.11.6311.
4
Hepatocyte nuclear factor 4 expression overcomes repression of the hepatic phenotype in dedifferentiated hepatoma cells.肝细胞核因子4的表达克服了去分化肝癌细胞中肝表型的抑制。
Mol Cell Biol. 1997 Apr;17(4):1913-22. doi: 10.1128/MCB.17.4.1913.
5
Genetic regulation of gene-specific mRNA by ethanol in vivo and its possible role in ethanol preference in a cross with RI lines in mice.乙醇对体内基因特异性mRNA的遗传调控及其在与小鼠RI品系杂交中对乙醇偏好的可能作用。
Biochem Genet. 1996 Jun;34(5-6):219-38. doi: 10.1007/BF02407021.
6
Amino acid substitution at the Adh locus of Drosophila is facilitated by small population size.果蝇乙醇脱氢酶（Adh）基因座上的氨基酸替换因种群规模小而更容易发生。
Proc Natl Acad Sci U S A. 1993 May 15;90(10):4548-51. doi: 10.1073/pnas.90.10.4548.
7
Molecular structure and genetic regulation of SFA, a gene responsible for resistance to formaldehyde in Saccharomyces cerevisiae, and characterization of its protein product.酿酒酵母中负责甲醛抗性的基因SFA的分子结构、遗传调控及其蛋白质产物的特性
Mol Gen Genet. 1993 Mar;237(3):351-8. doi: 10.1007/BF00279438.
8
Genetically determined differences in liver alcohol dehydrogenase activity in male rats.雄性大鼠肝脏乙醇脱氢酶活性的遗传决定差异。
Biochem Genet. 1985 Dec;23(11-12):987-96. doi: 10.1007/BF00499942.
9
Purification and characterization of the Danish (Skive) variant of mouse liver alcohol dehydrogenase.小鼠肝脏乙醇脱氢酶丹麦（斯基沃）变体的纯化与特性分析
Biochem Genet. 1987 Feb;25(1-2):111-21. doi: 10.1007/BF00498955.
10
Mammalian alcohol dehydrogenases of separate classes: intermediates between different enzymes and intraclass isozymes.不同类别的哺乳动物乙醇脱氢酶：不同酶之间的中间体和类内同工酶。
Proc Natl Acad Sci U S A. 1987 May;84(9):2580-4. doi: 10.1073/pnas.84.9.2580.

本文引用的文献

1
The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA.合成寡核苷酸作为杂交探针的应用。II. 混合序列寡核苷酸与兔β-珠蛋白DNA的杂交
Nucleic Acids Res. 1981 Feb 25;9(4):879-94. doi: 10.1093/nar/9.4.879.
2
In vivo chemical modification of proteins (post-translational modification).蛋白质的体内化学修饰（翻译后修饰）。
Annu Rev Biochem. 1981;50:783-814. doi: 10.1146/annurev.bi.50.070181.004031.
3
Initiation of translation at internal AUG codons in mammalian cells.哺乳动物细胞中内部AUG密码子处的翻译起始。
Nature. 1984;309(5963):82-5. doi: 10.1038/309082a0.
4
Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.真核生物mRNA翻译起始位点上游序列的汇编与分析。
Nucleic Acids Res. 1984 Jan 25;12(2):857-72. doi: 10.1093/nar/12.2.857.
5
A comprehensive sequence analysis program for the IBM personal computer.一款适用于IBM个人计算机的综合序列分析程序。
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):581-99. doi: 10.1093/nar/12.1part2.581.
6
Human liver alcohol dehydrogenase: amino acid substitution in the beta 2 beta 2 Oriental isozyme explains functional properties, establishes an active site structure, and parallels mutational exchanges in the yeast enzyme.人肝脏乙醇脱氢酶：β2β2东方同工酶中的氨基酸取代解释了功能特性，确立了活性位点结构，并与酵母酶中的突变交换相似。
Proc Natl Acad Sci U S A. 1984 May;81(10):3024-8. doi: 10.1073/pnas.81.10.3024.
7
Purification and characterization of mouse alcohol dehydrogenase from two inbred strains that differ in total liver enzyme activity.从两种总肝酶活性不同的近交系小鼠中纯化和鉴定乙醇脱氢酶。
Biochem Genet. 1984 Feb;22(1-2):115-24. doi: 10.1007/BF00499291.
8
Purification and molecular properties of mouse alcohol dehydrogenase isozymes.小鼠乙醇脱氢酶同工酶的纯化及分子特性
Eur J Biochem. 1983 Dec 1;137(1-2):139-47. doi: 10.1111/j.1432-1033.1983.tb07807.x.
9
Relationship between kinetics of liver alcohol dehydrogenase and alcohol metabolism.肝脏乙醇脱氢酶动力学与酒精代谢之间的关系。
Pharmacol Biochem Behav. 1983;18 Suppl 1:223-7. doi: 10.1016/0091-3057(83)90175-2.
10
Kinetic and electrophoretic properties of native and recombined isoenzymes of human liver alcohol dehydrogenase.人肝脏乙醇脱氢酶天然和重组同工酶的动力学及电泳性质
Biochemistry. 1983 Apr 12;22(8):1852-7. doi: 10.1021/bi00277a017.

编码完整小鼠肝脏乙醇脱氢酶的cDNA的克隆与测序

Cloning and sequencing of cDNA encoding the complete mouse liver alcohol dehydrogenase.

作者信息

Edenberg H J, Zhang K, Fong K, Bosron W F, Li T K

出版信息

Proc Natl Acad Sci U S A. 1985 Apr;82(8):2262-6. doi: 10.1073/pnas.82.8.2262.

DOI:10.1073/pnas.82.8.2262
PMID:3157987
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC397537/
Abstract

The main ethanol-active alcohol dehydrogenase (ADH; alcohol:NAD+ oxidoreductase, EC 1.1.1.1) in mouse liver (ADH-AA) is similar in catalytic and molecular properties to horse liver ADH-EE and to the human class I ADHs. We have isolated cDNA clones encoding the entire mouse liver enzyme plus flanking regions. A mixture of 16 different oligonucleotides, each 14 bases long, was used to screen a liver cDNA library made from a DBA/2J mouse. A strongly hybridizing clone was found and identified as an ADH-encoding cDNA by partial DNA sequencing. This clone was used as a probe to identify others. Two overlapping cDNA clones together contained the entire protein-encoding region plus 100 nucleotides of the 5' noncoding region and 133 nucleotides of the 3' noncoding region culminating in a short poly(dA) tail. The amino acid sequence of the mouse liver enzyme deduced from this cDNA closely resembles that of horse liver ADH-E: 316 of 374 residues are identical, and 29 of the differences are conservative substitutions. The 5' region of this cDNA is interesting: the AUG that initiates the ADH polypeptide is preceded by an AUG that would encode the first amino acid of a tripeptide. Presumably termination of this tripeptide is followed by reinitiation at the AUG immediately preceding the sequence of the mature ADH polypeptide.

摘要

小鼠肝脏中的主要乙醇活性醇脱氢酶（ADH；醇：NAD+氧化还原酶，EC 1.1.1.1）（ADH-AA）在催化和分子特性上与马肝脏ADH-EE以及人类I类ADH相似。我们已经分离出编码整个小鼠肝脏酶及其侧翼区域的cDNA克隆。使用16种不同的寡核苷酸混合物，每种寡核苷酸长度为14个碱基，来筛选由DBA/2J小鼠制备的肝脏cDNA文库。发现了一个强杂交克隆，并通过部分DNA测序将其鉴定为编码ADH的cDNA。这个克隆被用作探针来鉴定其他克隆。两个重叠的cDNA克隆一起包含了整个蛋白质编码区域，加上5'非编码区域的100个核苷酸和3'非编码区域的133个核苷酸，最终以一个短的聚（dA）尾巴结束。从这个cDNA推导的小鼠肝脏酶的氨基酸序列与马肝脏ADH-E非常相似：374个残基中有316个相同，29个差异是保守替换。这个cDNA的5'区域很有趣：启动ADH多肽的AUG之前还有一个AUG，它将编码一个三肽的第一个氨基酸。据推测，这个三肽的终止之后是在紧接成熟ADH多肽序列之前的AUG处重新起始。