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骨髓源性细胞在 GFP 小鼠骨髓移植模型中股骨肌肉异位骨形成中的作用。

The Role of Bone Marrow-Derived Cells during Ectopic Bone Formation of Mouse Femoral Muscle in GFP Mouse Bone Marrow Transplantation Model.

机构信息

Department of Oral Pathology and Medicine Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University, Okayama, Japan.

Department of life science, Faculty of Science, Okayama University of Science, Okayama, Japan.

出版信息

Int J Med Sci. 2018 May 22;15(8):748-757. doi: 10.7150/ijms.24605. eCollection 2018.

DOI:10.7150/ijms.24605
PMID:30008583
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6036083/
Abstract

Multipotential ability of bone marrow-derived cells has been clarified, and their involvement in repair and maintenance of various tissues has been reported. However, the role of bone marrow-derived cells in osteogenesis remains unknown. In the present study, bone marrow-derived cells during ectopic bone formation of mouse femoral muscle were traced using a GFP bone marrow transplantation model. Bone marrow cells from C57BL/6-Tg (CAG-EGFP) mice were transplanted into C57BL/6 J wild type mice. After transplantation, insoluble bone matrix (IBM) was implanted into mouse muscle. Ectopic bone formation was histologically assessed at postoperative days 7, 14, and 28. Immunohistochemistry for GFP single staining and GFP-osteocalcin double staining was then performed. Bone marrow transplantation successfully replaced hematopoietic cells with GFP-positive donor cells. Immunohistochemical analyses revealed that osteoblasts and osteocytes involved in ectopic bone formation were GFP-negative, whereas osteoclasts and hematopoietic cells involved in bone formation were GFP-positive. These results indicate that bone marrow-derived cells might not differentiate into osteoblasts. Thus, the main role of bone marrow-derived cells in ectopic osteogenesis may not be to induce bone regeneration by differentiation into osteoblasts, but rather to contribute to microenvironment formation for bone formation by differentiating tissue stem cells into osteoblasts.

摘要

骨髓细胞的多能性已经得到阐明，其在各种组织的修复和维持中的作用也已有报道。然而，骨髓细胞在成骨中的作用仍不清楚。在本研究中，我们使用 GFP 骨髓移植模型追踪了小鼠股骨肌肉异位骨形成过程中的骨髓来源细胞。将 C57BL/6-Tg（CAG-EGFP）小鼠的骨髓细胞移植到 C57BL/6 J 野生型小鼠中。移植后，将不溶性骨基质（IBM）植入小鼠肌肉中。在术后第 7、14 和 28 天进行异位骨形成的组织学评估。然后进行 GFP 单染色和 GFP-骨钙素双染色的免疫组织化学染色。骨髓移植成功地用 GFP 阳性供体细胞替代了造血细胞。免疫组织化学分析显示，参与异位骨形成的成骨细胞和骨细胞为 GFP 阴性，而参与骨形成的破骨细胞和造血细胞为 GFP 阳性。这些结果表明，骨髓来源细胞可能不会分化为成骨细胞。因此，骨髓来源细胞在异位成骨中的主要作用可能不是通过分化为成骨细胞来诱导骨再生，而是通过分化组织干细胞为成骨细胞来促进骨形成的微环境形成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9035/6036083/b76f61dc856b/ijmsv15p0748g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9035/6036083/73329964fe2a/ijmsv15p0748g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9035/6036083/359e5fc5e9c4/ijmsv15p0748g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9035/6036083/32aea9f617f0/ijmsv15p0748g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9035/6036083/b76f61dc856b/ijmsv15p0748g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9035/6036083/73329964fe2a/ijmsv15p0748g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9035/6036083/359e5fc5e9c4/ijmsv15p0748g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9035/6036083/32aea9f617f0/ijmsv15p0748g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9035/6036083/b76f61dc856b/ijmsv15p0748g007.jpg

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1
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2
Skeletal muscle-resident MSCs and bone formation.骨骼肌驻留间充质干细胞与骨形成。
Bone. 2015 Nov;80:19-23. doi: 10.1016/j.bone.2015.06.013. Epub 2015 Jun 21.
3
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Cell Biosci. 2022 May 26;12(1):70. doi: 10.1186/s13578-022-00813-7.
4
Ectopic Bone Tissue Engineering in Mice Using Human Gingiva or Bone Marrow-Derived Stromal/Progenitor Cells in Scaffold-Hydrogel Constructs.使用人牙龈或骨髓来源的基质/祖细胞在支架-水凝胶构建物中进行小鼠异位骨组织工程
Front Bioeng Biotechnol. 2021 Nov 30;9:783468. doi: 10.3389/fbioe.2021.783468. eCollection 2021.
5
Bone regeneration in rat calvarial defects using dissociated or spheroid mesenchymal stromal cells in scaffold-hydrogel constructs.使用支架-水凝胶构建物中的分离或球体状间充质基质细胞在大鼠颅骨缺损中进行骨再生。
Stem Cell Res Ther. 2021 Nov 14;12(1):575. doi: 10.1186/s13287-021-02642-w.
6
Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice.C57BL/6-Tg（CAG-EGFP）小鼠对全身照射的反应改变。
Dose Response. 2020 Aug 26;18(3):1559325820951332. doi: 10.1177/1559325820951332. eCollection 2020 Jul-Sep.
Calcif Tissue Int. 2013 Mar;92(3):296-306. doi: 10.1007/s00223-012-9685-3. Epub 2012 Dec 22.
4
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5
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10
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