胱天蛋白酶募集结构域（CARD）家族（CARD9、CARD10、CARD11、CARD14和CARD15）在炎症性肠病患者的活动性炎症期间会增加。
Caspase recruitment domain (CARD) family (CARD9, CARD10, CARD11, CARD14 and CARD15) are increased during active inflammation in patients with inflammatory bowel disease.
作者信息
Yamamoto-Furusho Jesús K, Fonseca-Camarillo Gabriela, Furuzawa-Carballeda Janette, Sarmiento-Aguilar Andrea, Barreto-Zuñiga Rafael, Martínez-Benitez Braulio, Lara-Velazquez Montserrat A
机构信息
1Department of Gastroenterology, Inflammatory Bowel Disease Clinic, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga 15, Colonia Sección XVI, Tlalpan, CP, 14000 Mexico City, Mexico.
2Department of Immunology and Rheumatology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico.
出版信息
J Inflamm (Lond). 2018 Jul 11;15:13. doi: 10.1186/s12950-018-0189-4. eCollection 2018.
BACKGROUND
The CARD family plays an important role in innate immune response by the activation of NF-κB. The aim of this study was to determine the gene expression and to enumerate the protein-expressing cells of some members of the CARD family (CARD9, CARD10, CARD11, CARD14 and CARD15) in patients with IBD and normal controls without colonic inflammation.
METHODS
We included 48 UC patients, 10 Crohn's disease (CD) patients and 18 non-inflamed controls. Gene expression was performed by RT-PCR and protein expression by immunohistochemistry. CARD-expressing cells were assessed by estimating the positively staining cells and reported as the percentage.
RESULTS
The CARD9 and CARD10 gene expression was significantly higher in UC groups compared with CD ( 0.001). CARD11 had lower gene expression in UC than in CD patients ( 0.001). CARD14 gene expression was higher in the group with active UC compared to non-inflamed controls ( 0.001). The low expression of CARD14 gene was associated with a benign clinical course of UC, characterized by initial activity followed by long-term remission longer than 5 years ( 0.01, OR = 0.07, 95%CI:0.007-0.70). CARD15 gene expression was lower in UC patients versus CD ( 0.004). CARD9 protein expression was detected in inflammatory infiltrates; CARD14 in parenchymal cells, while CARD15 in inflammatory and parenchymal cells. CARD9-, CARD14- and CARD15 - expressing cells were significantly higher in patients with active UC versus non-inflamed controls ( 0.05).
CONCLUSION
The CARD family is involved in the inflammatory process and might be involved in the IBD pathophysiology.
背景
CARD家族通过激活核因子κB在先天性免疫反应中发挥重要作用。本研究的目的是确定炎症性肠病（IBD）患者和无结肠炎症的正常对照中CARD家族某些成员（CARD9、CARD10、CARD11、CARD14和CARD15）的基因表达情况，并对表达蛋白的细胞进行计数。
方法
我们纳入了48例溃疡性结肠炎（UC）患者、10例克罗恩病（CD）患者和18例无炎症对照。通过逆转录聚合酶链反应（RT-PCR）检测基因表达，通过免疫组织化学检测蛋白表达。通过估计阳性染色细胞来评估表达CARD的细胞，并以百分比形式报告。
结果
与CD组相比，UC组中CARD9和CARD10基因表达显著更高（P<0.001）。UC患者中CARD11的基因表达低于CD患者（P<0.001）。与无炎症对照相比，活动期UC组中CARD14基因表达更高（P<0.001）。CARD14基因的低表达与UC的良性临床病程相关，其特征为初始活动期后有超过5年的长期缓解（P<0.01，OR=0.07，95%CI：0.007-0.70）。UC患者中CARD15基因表达低于CD患者（P<0.004）。在炎性浸润中检测到CARD9蛋白表达；在实质细胞中检测到CARD14，而在炎性和实质细胞中检测到CARD15。与无炎症对照相比，活动期UC患者中表达CARD9、CARD14和CARD15的细胞显著更高（P<0.05）。
结论
CARD家族参与炎症过程，可能与IBD的病理生理学有关。
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