在人类核基因组中存在的一个类似线粒体DNA的序列内插入一个长的KpnI家族成员。

Insertion of a long KpnI family member within a mitochondrial-DNA-like sequence present in the human nuclear genome.

作者信息

Wakasugi S, Nomiyama H, Fukuda M, Tsuzuki T, Shimada K

出版信息

Gene. 1985;36(3):281-8. doi: 10.1016/0378-1119(85)90183-0.

Abstract

Structural analysis of a phage lambda Charon 4A clone carrying one of the human nuclear mitochondrial(mut)-DNA-like sequences revealed that a KpnI-family member (KpnI 5.5-kb DNA) is inserted within this sequence. The inserted KpnI 5.5-kb DNA contains several possible polyadenylation signal sequences followed by an A-rich sequence at its 3' end and is flanked by perfect 13-bp direct repeats of the duplicated mtDNA-like sequences. These structures strongly suggest that the KpnI 5.5-kb DNA is a mobile element. Comparison of the 5' terminal sequences of the KpnI 5.5-kb DNA and four other long KpnI-family DNAs so far examined, using the predicted general promoter sequence for eukaryotic tRNAs, indicates that they contain the consensus sequences for the split internal RNA polymerase III control region.

摘要

对携带一种人类核线粒体（mut）-DNA样序列的噬菌体λCharon 4A克隆进行结构分析发现，一个KpnI家族成员（KpnI 5.5-kb DNA）插入到该序列中。插入的KpnI 5.5-kb DNA包含几个可能的聚腺苷酸化信号序列，其3'端接着一个富含A的序列，并且两侧是重复的线粒体DNA样序列的完美13-bp直接重复序列。这些结构强烈表明KpnI 5.5-kb DNA是一个可移动元件。使用预测的真核tRNA通用启动子序列，对KpnI 5.5-kb DNA的5'末端序列与目前已检测的其他四个长KpnI家族DNA进行比较，结果表明它们包含分裂型内部RNA聚合酶III控制区的共有序列。