Wang Aihong, Wang Mingquan, Pang Qiuxia, Jia Lei, Zhao Jumei, Chen Meini, Zhao Yufeng
School of Medicine, Yan'an University, Yan'an, Shaanxi 716000, P.R. China.
Department of Interventional Radiology, Affiliated Hospital of Yan'an University, Yan'an, Shaanxi 716000, P.R. China.
Oncol Lett. 2018 Aug;16(2):1397-1404. doi: 10.3892/ol.2018.8806. Epub 2018 May 24.
The present study aimed to investigate the effect of alkaloids and carbinol extracts from lily on the proliferation of SGC-7901 cells, as well as the underlying mechanism. SGC-7901 cells were incubated with different concentrations of alkaloid or carbinol extracts for 24, 48 or 72 h. MTT assays were used to measure the inhibition rate of SGC-7901 cell proliferation. Inverted phase contrast and fluorescence microscopy was used to observe morphological changes of SGC-7901 cells. Flow cytometry was employed to detect cell cycle progression and apoptosis rates of SGC-7901 cells. Western blotting was performed to measure the expression of caspase-3, Fas and Fas ligand (FasL) proteins in SGC-7901 cells. The inhibition rate of SGC-7901 cell proliferation was significantly enhanced with increasing drug concentrations and time elapsed. Treatment with alkaloid or carbinol extracts deteriorated the morphology of SGC-7901 cells in a dose-dependent manner. Alkaloid and carbinol extracts arrested SGC-7901 cells in the G/M phase, and induced apoptosis in a dose-dependent manner. Alkaloid and carbinol extracts enhanced caspase-3, and Fas expression, but reduced FasL expression in SGC-7901 cells. The present study demonstrated that alkaloids and carbinol extracts from lily inhibited the proliferation of gastric carcinoma SGC-7901 cells by arresting cells in the G/M phase. The upregulation of caspase-3 and Fas proteins, and the downregulation of FasL protein may be an important mechanism for the induction of SGC-7901 cell apoptosis.
本研究旨在探讨百合生物碱及甲醇提取物对SGC - 7901细胞增殖的影响及其潜在机制。将SGC - 7901细胞与不同浓度的生物碱或甲醇提取物孵育24、48或72小时。采用MTT法检测SGC - 7901细胞增殖抑制率。利用倒置相差显微镜和荧光显微镜观察SGC - 7901细胞的形态变化。采用流式细胞术检测SGC - 7901细胞的细胞周期进程和凋亡率。通过蛋白质免疫印迹法检测SGC - 7901细胞中caspase - 3、Fas和Fas配体（FasL）蛋白的表达。随着药物浓度的增加和时间的延长，SGC - 7901细胞增殖抑制率显著提高。生物碱或甲醇提取物处理使SGC - 7901细胞形态呈剂量依赖性恶化。生物碱和甲醇提取物使SGC - 7901细胞停滞于G/M期，并呈剂量依赖性诱导细胞凋亡。生物碱和甲醇提取物增强了SGC - 7901细胞中caspase - 3和Fas的表达，但降低了FasL的表达。本研究表明，百合生物碱和甲醇提取物通过使细胞停滞于G/M期抑制胃癌SGC - 7901细胞的增殖。caspase - 3和Fas蛋白的上调以及FasL蛋白的下调可能是诱导SGC - 7901细胞凋亡的重要机制。
