Wang Guan-Nan, Zhong Ming, Chen Yv, Ji Jia, Gao Xiu-Qiu, Wang Tian-Fu
Department of Pathology, Stomatological Hospital of China Medical University, Shenyang, Liaoning 110002, P.R. China.
Basic Medicine College, Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China.
Oncol Lett. 2018 Aug;16(2):1507-1512. doi: 10.3892/ol.2018.8820. Epub 2018 May 25.
The present study aimed to measure the expression of WNT1 in ameloblastoma (AB). Immunohistochemistry was used to observe changes in WNT1 expression in 80 AB samples, 10 keratocystic odontogenic tumor (KCOT) samples and 10 normal oral mucosa (NOM) samples. Western blotting and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) were used to measure WNT1 protein and mRNA expression, respectively, in 30 AB samples, 5 KCOT samples, 5 NOM samples and 3 tooth germ samples. Ectopic cytoplasmic expression of WNT1 was detected in AB; 88.8% (71/80) of the samples were WNT1-positive. The western blotting results demonstrated that compared with NOM (0.57±0.05), WNT1 expression was significantly higher in AB tissue (1.74±0.36, P0.05), whereas it was not significantly different between AB and KCOT samples (0.80±0.06, P0.05). RT-qPCR revealed that the level of gene expression in AB was increased 2.43-fold compared with normal mucosa, and 1.77-fold compared with tooth germ tissue. In conclusion, WNT1 protein and mRNA expression were increased in AB, and there was ectopic cytoplasmic expression. This indicates that WNT1 may serve an important role in AB occurrence and development.
本研究旨在检测WNT1在成釉细胞瘤（AB）中的表达。采用免疫组织化学方法观察80例AB样本、10例牙源性角化囊性瘤（KCOT）样本和10例正常口腔黏膜（NOM）样本中WNT1表达的变化。分别采用蛋白质免疫印迹法和逆转录定量聚合酶链反应（RT-qPCR）检测30例AB样本、5例KCOT样本、5例NOM样本和3例牙胚样本中WNT1蛋白和mRNA的表达。在AB中检测到WNT1的异位胞质表达；88.8%（71/80）的样本WNT1呈阳性。蛋白质免疫印迹结果显示，与NOM（0.57±0.05）相比，AB组织中WNT1表达显著升高（1.74±0.36，P<0.05），而AB与KCOT样本之间无显著差异（0.80±0.06，P>0.05）。RT-qPCR显示，与正常黏膜相比，AB中基因表达水平增加了2.43倍，与牙胚组织相比增加了1.77倍。总之，AB中WNT1蛋白和mRNA表达增加，且存在异位胞质表达。这表明WNT1可能在AB的发生和发展中起重要作用。
