Yang Weili, Luo Yongsheng, Hu Shuangcheng, Li Ying, Liu Qing
Department of Gastroenterology, The Second People's Hospital of Shenzhen, Shenzhen, Guangdong 518000, P.R. China.
Oncol Lett. 2018 Aug;16(2):1551-1556. doi: 10.3892/ol.2018.8792. Epub 2018 May 24.
The aim of the present study was to investigate the value of combined detection of serum carcino-embryonic antigen (CEA), carbohydrate antigen (CA) 19-9 and cyclooxygenase-2 (COX-2) in the diagnosis of colorectal cancer. A total of 50 patients with colorectal cancer were selected as Group A and 50 healthy subjects as the control group. A sample of 2 ml fasting venous blood was drawn from patients in each group, and serum CEA, CA19-9 and COX-2 were detected using electrochemiluminescence analyzer and ELISA. Receiver operating characteristic curve analysis was performed on analyze the sensitivity and specificity of diagnostic methods for colorectal cancer patients at different stages. The expression levels of CEA, CA199 and COX-2 in the cancer patients group were significantly higher than those in the healthy group (P<0.05). The coincidence rates of CEA, CA199, COX-2 and combined detection were 56.0, 64.0, 62.0 and 88.0%, respectively. The coincidence rate of combined detection was significantly higher than that of diagnosis using a single factor (P<0.05). Sensitivity of combined detection of colorectal cancer patients with stage I, II, III and IV were 82.9, 85.3, 86.4 and 88.7%, respectively. The specificities were 65.3, 68.7, 57.8 and 58.6%, respectively. Thus, CEA, CA199 and COX-2 in serum are highly expressed in colorectal cancer patients, and may useful as effective indicators for the early diagnosis of colorectal cancer.
本研究旨在探讨联合检测血清癌胚抗原（CEA）、糖类抗原（CA）19-9和环氧化酶-2（COX-2）在结直肠癌诊断中的价值。选取50例结直肠癌患者作为A组，50例健康受试者作为对照组。采集每组患者2ml空腹静脉血，采用电化学发光分析仪和酶联免疫吸附测定法检测血清CEA、CA19-9和COX-2。绘制受试者工作特征曲线分析不同分期结直肠癌患者诊断方法的敏感性和特异性。癌症患者组中CEA、CA199和COX-2的表达水平显著高于健康组（P<0.05）。CEA、CA199、COX-2及联合检测的符合率分别为56.0%、64.0%、62.0%和88.0%。联合检测的符合率显著高于单因素诊断（P<0.05）。I、II、III和IV期结直肠癌患者联合检测的敏感性分别为82.9%、85.3%、86.4%和88.7%。特异性分别为65.3%、68.7%、57.8%和58.6%。因此，血清中的CEA、CA199和COX-2在结直肠癌患者中高表达，可能作为结直肠癌早期诊断的有效指标。
