Liu Wei, Wan Xiuwei, Mu Zongyun, Li Fei, Wang Lei, Zhao Jing, Huang Xiaori
Department of Respiratory Medicine, People's Hospital of Rizhao, Rizhao, Shandong 276826, P.R. China.
Oncol Lett. 2018 Aug;16(2):1708-1714. doi: 10.3892/ol.2018.8794. Epub 2018 May 24.
Mounting evidence has shown that miRNA expression is abnormal in various human cancers. Here, we mainly explored the biological function and the potential mechanisms of miR-1256 in non-small cell lung cancer (NSCLC). The miR-1256 mRNA expression was detected by quantitative real-time PCR and tectonic family member 1 (TCTN1) mRNA expression was detected by immunoblotting. The TCTN1 was identified to be the direct and specific target gene of miR-1256 by luciferase reporter assay. Cell proliferation was examined by methyl thiazolyl tetrazolium assay and migration was detected by transwell assay. MiR-1256 expression was downregulated in NSCLC tissues, whereas the expression of TCTN1 was upregulated, compared with normal tissues. We also found that overexpression of miR-1256 in these NSCLC cell lines inhibited cell proliferation and migration. Furthermore, TCTN1 was identified as a direct target of miR-1256 by luciferase reporter assays. Collectively, these data stated that the inhibitory effect of miR-1256 in NSCLC was realized by upregulating TCTN1, suggesting that miR-1256/TCTN1 axis may play a critical role as NSCLC therapeutic target.
越来越多的证据表明，miRNA表达在各种人类癌症中均存在异常。在此，我们主要探讨了miR-1256在非小细胞肺癌（NSCLC）中的生物学功能及潜在机制。通过定量实时PCR检测miR-1256 mRNA表达，通过免疫印迹检测tectonic家族成员1（TCTN1）mRNA表达。通过荧光素酶报告基因检测确定TCTN1是miR-1256的直接且特异性靶基因。采用甲基噻唑基四氮唑法检测细胞增殖，通过Transwell检测迁移。与正常组织相比，NSCLC组织中miR-1256表达下调，而TCTN1表达上调。我们还发现，在这些NSCLC细胞系中过表达miR-1256可抑制细胞增殖和迁移。此外，通过荧光素酶报告基因检测确定TCTN1是miR-1256的直接靶标。总体而言，这些数据表明，miR-1256在NSCLC中的抑制作用是通过上调TCTN1实现的，这表明miR-1256/TCTN1轴可能作为NSCLC治疗靶点发挥关键作用。
