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微小RNA-205-5p通过靶向锌指E盒结合蛋白1抑制前列腺癌细胞的迁移和侵袭。

miR-205-5p inhibits cell migration and invasion in prostatic carcinoma by targeting ZEB1.

作者信息

Li Lianpeng, Li Shouqiang

机构信息

Department of Urological Surgery, Binzhou Central Hospital, Binzhou, Shandong 251700, P.R. China.

Department of Urological Surgery, People's Hospital of Dongying District, Dongying, Shandong 257000, P.R. China.

出版信息

Oncol Lett. 2018 Aug;16(2):1715-1721. doi: 10.3892/ol.2018.8862. Epub 2018 May 31.

DOI:10.3892/ol.2018.8862
PMID:30008858
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6036508/
Abstract

Many studies have demonstrated that miRNAs have influence on tumorigenesis and progression of human cancers, including invasion and migration. Thus, the role of miR-205/ZEB1 axis for the migration and invasion of prostate cancer cells was explored in the present study. The miR-205-5p and zinc finger E-box binding homeobox 1 (ZEB1) mRNA expression levels were observed in prostate cancer tissues or cell lines via reverse transcription-quantitative PCR (RT-qPCR), and the protein level of ZEB1 was measured by western blotting. Dual-Luciferase Reporter Assay was used to verify the relationship between miR-205-5p and ZEB1. In addition, cell migration and invasion was measured by Transwell assay. The results revealed that, compared with the control, downregulation of miR-205-5p was detected in prostate cancer tissues and cell lines, and miR-205-5p overexpression was found to inhibit cell migration and invasion. Moreover, miR-205-5p was confirmed to directly target ZEB1 in prostate cancer. Importantly, ZEB1 was identified to weaken the inhibitory effect of miR-205-5p in prostate cancer. In conclusion, miR-205-5p inhibited cell migration and invasion in prostatic carcinoma by targeting ZEB1 and miR-205-5p/ZEB1 axis shows potential to be developed in therapeutic strategies for prostate cancer.

摘要

许多研究表明，微小RNA（miRNAs）对人类癌症的发生和发展有影响，包括侵袭和迁移。因此，本研究探讨了miR-205/ZEB1轴在前列腺癌细胞迁移和侵袭中的作用。通过逆转录定量PCR（RT-qPCR）观察前列腺癌组织或细胞系中miR-205-5p和锌指E盒结合同源框1（ZEB1）mRNA的表达水平，并用蛋白质印迹法检测ZEB1的蛋白水平。采用双荧光素酶报告基因检测法验证miR-205-5p与ZEB1之间的关系。此外，通过Transwell检测法测定细胞迁移和侵袭能力。结果显示，与对照组相比，前列腺癌组织和细胞系中miR-205-5p表达下调，过表达miR-205-5p可抑制细胞迁移和侵袭。此外，证实miR-205-5p在前列腺癌中直接靶向ZEB1。重要的是，发现ZEB1可削弱miR-205-5p在前列腺癌中的抑制作用。总之，miR-205-5p通过靶向ZEB1抑制前列腺癌细胞的迁移和侵袭，miR-205-5p/ZEB1轴在前列腺癌治疗策略中具有开发潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92ca/6036508/bf1de56e11ff/ol-16-02-1715-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92ca/6036508/a185b2fde678/ol-16-02-1715-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92ca/6036508/6be3826ca76f/ol-16-02-1715-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92ca/6036508/39384c1d2be1/ol-16-02-1715-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92ca/6036508/fcac87d894f6/ol-16-02-1715-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92ca/6036508/bf1de56e11ff/ol-16-02-1715-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92ca/6036508/a185b2fde678/ol-16-02-1715-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92ca/6036508/6be3826ca76f/ol-16-02-1715-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92ca/6036508/39384c1d2be1/ol-16-02-1715-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92ca/6036508/fcac87d894f6/ol-16-02-1715-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92ca/6036508/bf1de56e11ff/ol-16-02-1715-g04.jpg

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