Single-cell and bulk RNA sequencing data jointly reveals s impacts on prognosis and immune landscape of NSCLC.

Non-small cell lung cancer (NSCLC) is characterized by stronger metastatic ability and worse prognosis. In NSCLC, hypoxia is a major cause of invasion and metastasis through promoting angiogenesis. In present study, NSCLC cell clusters were extracted from single cell-sequencing dataset GSE131907, which were combined with hypoxia-related genes to group clusters. qRT-PCR and western blot were used to validate the expression of target gene. Nine NSCLC clusters were extracted, which were divided into two hypoxia-related subgroups, C1 and C2. Totally 101 differentially expressed prognostic genes were identified between subgroups. Of which, showed excellent prognostic value for NSCLC and was selected for further analysis. was upregulated in tumor samples in TCGA and was correlated with advanced stages. experiments validated this trend. Five crucial immune cells showed differential infiltration proportions between high and low expression groups. knockdown significantly inhibited the proliferation and invasion ability of NSCLC cells. Integrating single cell and bulk sequencing data as well as wet lab experiments, hypoxia-related exhibited important prognostic value and showed the promise of becoming immune-therapy target in NSCLC.