Department of Thoracic Surgery, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010059, Inner Mongolia Autonomous Region, China.
Department of Thoracic Surgery, Peking University Cancer Hospital (Inner Mongolia Campus) and Affiliated Cancer Hospital of Inner Mongolia Medical University, Hohhot 010020, Inner Mongolia Autonomous Region, China.
Aging (Albany NY). 2024 Feb 20;16(4):3160-3184. doi: 10.18632/aging.205517.
Non-small cell lung cancer (NSCLC) is characterized by stronger metastatic ability and worse prognosis. In NSCLC, hypoxia is a major cause of invasion and metastasis through promoting angiogenesis. In present study, NSCLC cell clusters were extracted from single cell-sequencing dataset GSE131907, which were combined with hypoxia-related genes to group clusters. qRT-PCR and western blot were used to validate the expression of target gene. Nine NSCLC clusters were extracted, which were divided into two hypoxia-related subgroups, C1 and C2. Totally 101 differentially expressed prognostic genes were identified between subgroups. Of which, showed excellent prognostic value for NSCLC and was selected for further analysis. was upregulated in tumor samples in TCGA and was correlated with advanced stages. experiments validated this trend. Five crucial immune cells showed differential infiltration proportions between high and low expression groups. knockdown significantly inhibited the proliferation and invasion ability of NSCLC cells. Integrating single cell and bulk sequencing data as well as wet lab experiments, hypoxia-related exhibited important prognostic value and showed the promise of becoming immune-therapy target in NSCLC.
非小细胞肺癌（NSCLC）的特点是转移能力较强，预后较差。在 NSCLC 中，缺氧是通过促进血管生成促进侵袭和转移的主要原因。在本研究中，从单细胞测序数据集 GSE131907 中提取了 NSCLC 细胞簇，将其与与缺氧相关的基因相结合进行聚类。qRT-PCR 和 Western blot 用于验证靶基因的表达。提取了 9 个 NSCLC 簇，分为与缺氧相关的两个亚群 C1 和 C2。总共鉴定出 101 个亚组间差异表达的预后基因。其中，在 TCGA 中，表现出对 NSCLC 优异的预后价值，并被选择进行进一步分析。在 TCGA 中，在肿瘤样本中上调，并与晚期相关。实验验证了这一趋势。五种关键免疫细胞在高低 表达组之间表现出不同的浸润比例。敲低显著抑制 NSCLC 细胞的增殖和侵袭能力。整合单细胞和批量测序数据以及湿实验室实验，缺氧相关的 表现出重要的预后价值，并有望成为 NSCLC 的免疫治疗靶点。
