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酿酒酵母与大肠杆菌光复活基因之间的同源性。

Homology between the photoreactivation genes of Saccharomyces cerevisiae and Escherichia coli.

作者信息

Yasui A, Langeveld S A

出版信息

Gene. 1985;36(3):349-55. doi: 10.1016/0378-1119(85)90190-8.

DOI:10.1016/0378-1119(85)90190-8
PMID:3000886
Abstract

A cloned fragment of Saccharomyces cerevisiae chromosomal DNA carrying the photoreactivation gene (PHR) has been sequenced. The fragment contains a 1695-bp intronless open reading frame (ORF) coding for a polypeptide of 564 amino acids (aa). The phr gene of Escherichia coli was also sequenced, and the sequence is in agreement with the published data. The yeast PHR gene has a G + C content of 36.2%, whereas 53.7% was found for the E. coli gene. Despite the difference in G + C content there is a 35% homology between the deduced aa sequences. This homology suggests that both genes have originated from a common ancestral gene.

摘要

一个携带光复活基因(PHR)的酿酒酵母染色体DNA克隆片段已被测序。该片段包含一个1695碱基对的无内含子开放阅读框(ORF),编码一个由564个氨基酸(aa)组成的多肽。大肠杆菌的phr基因也已测序,其序列与已发表的数据一致。酵母PHR基因的G + C含量为36.2%,而大肠杆菌基因的G + C含量为53.7%。尽管G + C含量存在差异,但推导的氨基酸序列之间有35%的同源性。这种同源性表明这两个基因起源于一个共同的祖先基因。

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引用本文的文献

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Nucleic Acids Res. 1985 Nov 25;13(22):8231-46. doi: 10.1093/nar/13.22.8231.
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