Curtis P J, Palumbo A, Ming J, Fraser P, Cioe L, Meo P, Shane S, Rovera G
Gene. 1985;36(3):357-62. doi: 10.1016/0378-1119(85)90191-x.
The results of hybridization analyses using cDNA probes for mouse and human alpha-spectrin mRNA indicate that a single gene encodes the alpha-subunit of erythrocyte spectrin. Sequencing of the cDNA clones showed that they code for 370 amino acids (aa) covering three repeat domains close to the C terminus of alpha-spectrin. The cloned cDNAs will now permit the isolation of the alpha-spectrin gene and should lead to the characterization of the genetic aspects in human hereditary anemias in which alpha-spectrin has been characterized as the site of the molecular defect.
使用针对小鼠和人类α-血影蛋白mRNA的cDNA探针进行杂交分析的结果表明，单个基因编码红细胞血影蛋白的α亚基。cDNA克隆的测序显示，它们编码370个氨基酸，覆盖α-血影蛋白C末端附近的三个重复结构域。克隆的cDNA现在将有助于分离α-血影蛋白基因，并应能对人类遗传性贫血的遗传方面进行表征，在这些贫血中，α-血影蛋白已被确定为分子缺陷的位点。
