Shih Wen-Ling, Chang Ching-Dong, Chen Hsiu-Tsu, Fan Kuo-Kuang
Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan, R.O.C.
Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan, R.O.C.
Oncol Lett. 2018 Aug;16(2):2046-2052. doi: 10.3892/ol.2018.8864. Epub 2018 May 31.
-acetyl-L-cysteine (NAC) is the most abundant water-soluble component of garlic. No study to date has studied the leukemia prevention ability of NAC in mouse systemic leukemia model. The current study aimed to investigate the leukemia initiation prevention potential of NAC in a mouse model. The cytotoxic concentration of NAC was determined first in HL-60 cells, and its activity was studied in a mouse acute myelocytic leukemia model with WEHI-3 leukemia cells. The results showed that a non-toxic concentration of NAC efficiently scavenged free-radicals, lowered lipid peroxidation and reduced DNA damage induced by hydrogen peroxide in a cultured HL-60 leukemia cell line. NAC also elevated the cellular antioxidant enzyme activity significantly. Furthermore, NAC prevented mouse death induced by injection of murine WEHI-3 leukemia cells and reduced organ damage, as well as activated antioxidant mechanisms. The results of this study provided strong evidence that NAC may have potential benefits in terms of elevating antioxidant activity and preventing leukemia initiation.
乙酰半胱氨酸（NAC）是大蒜中含量最丰富的水溶性成分。迄今为止，尚无研究在小鼠全身性白血病模型中研究NAC预防白血病的能力。当前的研究旨在调查NAC在小鼠模型中预防白血病起始的潜力。首先在HL-60细胞中确定NAC的细胞毒性浓度，并在携带WEHI-3白血病细胞的小鼠急性髓细胞白血病模型中研究其活性。结果表明，无毒浓度的NAC能有效清除自由基，降低脂质过氧化，并减少过氧化氢在培养的HL-60白血病细胞系中诱导的DNA损伤。NAC还显著提高了细胞抗氧化酶活性。此外，NAC可预防注射鼠源WEHI-3白血病细胞诱导的小鼠死亡，减少器官损伤，并激活抗氧化机制。这项研究的结果提供了强有力的证据，表明NAC在提高抗氧化活性和预防白血病起始方面可能具有潜在益处。
