卡特利链霉菌温和噬菌体TG1的分离与鉴定

Isolation and characterization of the Streptomyces cattleya temperate phage TG1.

作者信息

Foor F, Roberts G P, Morin N, Snyder L, Hwang M, Gibbons P H, Paradiso M J, Stotish R L, Ruby C L, Wolanski B

出版信息

Gene. 1985;39(1):11-6. doi: 10.1016/0378-1119(85)90101-5.

Abstract

A temperate actinophage, TG1, was isolated from soil by growth on Streptomyces cattleya and has been shown to be potentially useful for the cloning of DNA in this organism and other streptomycetes. It forms stable lysogens by integration at a unique site on the chromosome. The phage genome consists of 41 kb of double-stranded DNA with cohesive ends. It has unique sites for ClaI, NdeI, PstI, SmaI, and XbaI. The PstI site has been shown to be in a dispensable region of the phage genome. Deletions (2 kb in length) were obtained which retain this site and should be useful for the cloning of DNA.

摘要

一种温和型肌动噬菌体TG1，通过在卡特利链霉菌上生长从土壤中分离得到，并且已证明其在该生物体和其他链霉菌中对DNA克隆具有潜在用途。它通过整合到染色体上的一个独特位点形成稳定的溶原菌。噬菌体基因组由41 kb的双链DNA组成，两端具有粘性末端。它具有ClaI、NdeI、PstI、SmaI和XbaI的独特位点。已证明PstI位点位于噬菌体基因组的一个非必需区域。获得了长度为2 kb的缺失片段，这些片段保留了该位点，应该对DNA克隆有用。