Division of Hematology/Oncology, Department of Pediatrics, Children's Hospital of Michigan, Detroit, Michigan.
Department of Pediatrics, Wayne State University, Detroit, Michigan.
Pediatr Blood Cancer. 2018 Nov;65(11):e27307. doi: 10.1002/pbc.27307. Epub 2018 Jul 15.
The role of flow cytometry in diagnosis and management of Hodgkin lymphoma (HL) remains limited. As knowledge emerges of the tumor microenvironment in this disease, various methods are being evaluated in its study. This study examines the microenvironment using flow cytometry to assess differences between subtypes and clinicopathologic correlates.
A retrospective cross-sectional study was performed analyzing the tumor immunophenotype, by flow cytometry, for 31 children with classical HL. Correlation was made with patient information, including outcome.
The makeup of the tumor microenvironment varies across subtype of HL, with T cells predominating in nodular sclerosis (NS), and similar proportions of B and T cells in mixed cellularity (MC). CD4 cells predominate in NS, whereas CD8 more so in MC subtype. The rate of continuous complete remission is significantly higher in the MC subgroup. Last, the proportion of HLA-DR/CD38 copositive lymphocytes was an independent prognostic factor for relapse/refractoriness.
This study indicates that flow cytometry can be used to examine the tumor microenvironment in HL and that percentage of HLA-DR/CD38 copositive lymphocytes may be a biomarker for relapse and refractoriness in pediatric HL.
流式细胞术在霍奇金淋巴瘤（HL）的诊断和治疗中的作用仍然有限。随着对该病肿瘤微环境知识的不断涌现，各种方法正在对其进行研究。本研究使用流式细胞术来评估微环境，以评估亚型之间的差异及其与临床病理相关性。
回顾性横断面研究分析了 31 例经典 HL 患儿的肿瘤免疫表型，采用流式细胞术。并与患者信息，包括结局进行了相关性分析。
HL 的不同亚型中肿瘤微环境的组成不同，结节性硬化型（NS）以 T 细胞为主，混合细胞型（MC）中 B 和 T 细胞比例相似。NS 中 CD4 细胞占优势，而 MC 亚型中 CD8 细胞占优势。MC 亚组持续完全缓解率显著更高。此外，HLA-DR/CD38 共阳性淋巴细胞的比例是复发/难治的独立预后因素。
本研究表明流式细胞术可用于检查 HL 的肿瘤微环境，HLA-DR/CD38 共阳性淋巴细胞的比例可能是儿科 HL 复发和难治的生物标志物。
