Batista Jonas Dantas, Justino Oliveira Limirio Pedro Henrique, Rocha Flaviana Soares, Gomes Moura Camilla Christian, Zanetta-Barbosa Darceny, Dechichi Paula
Professor, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of Uberlândia, Uberlândia, Brazil.
Postdoctoral, Department of Histology, Faculty of Dentistry, University of Uberlândia, Uberlândia, Brazil.
J Oral Maxillofac Surg. 2018 Oct;76(10):2097-2102. doi: 10.1016/j.joms.2018.06.024. Epub 2018 Jun 27.
The aim of this study was to evaluate the effect of different storage media on angiogenesis and maintaining autogenous bone graft volume in rabbits.
Two grafts were removed bilaterally from the calvaria of 18 rabbits. One graft was removed and immediately fixed in the right mandibular angle (control group). The other graft was stored for 30 minutes in 1 of the following storage media (n = 6): saline solution (saline group), air exposure (dry group), or platelet-poor plasma (PPP group) and then retained by a screw in the right mandibular angle in the same animal. Four weeks later the animals were euthanized, and the grafted areas were harvested, fixed in 10% phosphate buffered formaldehyde solution, and embedded in paraffin. The 5-μm semi-serial sections were stained in hematoxylin and eosin and Mallory trichrome.
Histologic analysis of all groups showed the bone graft was vascularized and well incorporated into the recipient site. The number of blood vessels decreased in the saline and dry groups compared with the control group (P < .03); in contrast, the number of blood vessels increased in the PPP group (P < .05). There were fewer osteoclasts in the saline group compared with the control group (P < .05). Furthermore, the saline group showed larger numbers of blood vessels than the dry group (P < .01). The PPP group showed larger bone graft volumes compared with the dry and saline groups (P < .01). In addition, the saline group showed larger bone graft volumes than the dry group (P < .01).
PPP improved angiogenesis, maintained the volume of the autogenous bone graft, and was a better storage medium during the trans-surgical period than the dry and saline media.
本研究旨在评估不同储存介质对兔血管生成及自体骨移植体积维持的影响。
从18只兔的颅骨双侧取下两块移植物。一块移植物取出后立即固定于右侧下颌角（对照组）。另一块移植物在以下一种储存介质中储存30分钟（n = 6）：生理盐水溶液（生理盐水组）、暴露于空气中（干燥组）或乏血小板血浆（PPP组），然后用螺钉固定于同一只动物的右侧下颌角。4周后对动物实施安乐死，采集移植区域，固定于10%磷酸盐缓冲甲醛溶液中，然后石蜡包埋。5μm半连续切片进行苏木精-伊红染色和马洛里三色染色。
所有组的组织学分析显示骨移植血管化良好且与受体部位融合良好。与对照组相比，生理盐水组和干燥组的血管数量减少（P <.03）；相反，PPP组的血管数量增加（P <.05）。与对照组相比，生理盐水组的破骨细胞较少（P <.05）。此外，生理盐水组的血管数量多于干燥组（P <.01）。与干燥组和生理盐水组相比，PPP组的骨移植体积更大（P <.01）。另外，生理盐水组的骨移植体积大于干燥组（P <.01）。
PPP可改善血管生成，维持自体骨移植的体积，在手术期间是比干燥和生理盐水介质更好的储存介质。
