Dechichi Paula, Moura Camilla Christian Gomes, Santana Sandro Izaíras, Zanetta-Barbosa Darceny
School of Dentistry, University of Uberlandia, Minas Gerais, Brazil.
Int J Oral Maxillofac Implants. 2007 Nov-Dec;22(6):905-10.
In reconstructive procedures harvested bone grafts are often temporarily stored in extraoral media while the recipient site is prepared. The aim of the present study was to analyze histomorphometrically osteocytes in calvarial bone grafts stored in either physiologic saline solution or platelet-poor plasma (PPP).
Calvarial bone fragments were obtained from 12 rabbits and fixed immediately in formalin (control) or stored in PPP or in saline solution for 30 minutes prior to fixation. All specimens were decalcified and embedded in glycol methacrylate. A differential osteocyte count (normal osteocytes, abnormal osteocytes, and empty lacunae) was performed for the sections and submitted to analysis of variance (ANOVA) and the Fisher least-square-difference (LSD) test. P < .05 was considered significant.
The histomorphometric analysis demonstrated statistically significant differences among the groups (control, PPP, saline) for all analyzed parameters (P < .05). The median number of normal osteocytes observed was 31.8 +/- 2.3 for the control group, 29.7 +/- 4.2 for the PPP group, and 19.1 +/- 4.6 for the saline group. The median number of abnormal osteocytes observed was 14.7 +/- 3.9 for the saline group, 8.7 +/- 2.3 for the PPP group, and 7.0 +/- 2.3 for the control group. The median number of empty lacunae observed was 12.9 +/- 4.7 for the saline group, 8.5 +/- 2.4 for the PPP group, and 6.9 +/- 2.3 for the control group.
The current study has shown that PPP is a better storage medium for osteocyte preservation than physiologic saline solution. The best results were observed in the control group. (More than 50 references.)
在重建手术中，获取的骨移植材料在准备受区时通常会临时保存在口腔外的介质中。本研究的目的是通过组织形态计量学分析存储在生理盐溶液或贫血小板血浆（PPP）中的颅骨移植骨中的骨细胞。
从12只兔子获取颅骨碎片，立即用福尔马林固定（对照组），或在固定前在PPP或盐溶液中保存30分钟。所有标本脱钙并包埋在甲基丙烯酸乙二醇酯中。对切片进行差异骨细胞计数（正常骨细胞、异常骨细胞和空陷窝），并进行方差分析（ANOVA）和Fisher最小二乘差异（LSD）检验。P <.05被认为具有统计学意义。
组织形态计量学分析显示，所有分析参数在各组（对照组、PPP组、盐溶液组）之间均存在统计学显著差异（P <.05）。观察到的正常骨细胞中位数在对照组为31.8±2.3，PPP组为29.7±4.2，盐溶液组为19.1±4.6。观察到的异常骨细胞中位数在盐溶液组为14.7±3.9，PPP组为8.7±2.3，对照组为7.0±2.3。观察到的空陷窝中位数在盐溶液组为12.9±4.7，PPP组为8.5±2.4，对照组为6.9±2.3。
本研究表明，与生理盐溶液相比，PPP是一种更好的用于保存骨细胞的存储介质。对照组的结果最佳。（超过50篇参考文献。）
