黄芪醇提多糖的制备、特性及抗肿瘤活性。
Alcohol-soluble polysaccharide from Astragalus membranaceus: Preparation, characteristics and antitumor activity.
机构信息
Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; QingYunTang Biotech (Beijing) Co., Ltd. Beijing 100176, China.
Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
出版信息
Int J Biol Macromol. 2018 Oct 15;118(Pt B):2057-2064. doi: 10.1016/j.ijbiomac.2018.07.073. Epub 2018 Jul 14.
The alcohol-soluble polysaccharide (ASP) was extracted from Astragalus membranaceus, and their preliminary structural characteristics and in vivo antitumor activity were investigated in this study. The contents of total sugar, protein and uronic acid in ASP was 92.04%, 0.51% and 1.42%, respectively. FTIR and IC results indicated that ASP (about 2.1 × 10 Da) was a neutral polysaccharide composed of arabinose, galactose, glucose and mannose (molar ratio: 1.00:0.98:3.01:1.52) with pyranose ring and α-type glycosidic linkages. Besides, ASP could significantly inhibit the growth of H22 heptoma cells in vivo via improving the levels of serum cytokines (TNF-α, IL-2 and IFN-γ) and activities of immune cells (macrophages, lymphocytes and NK cells), thereby inducing tumor cell apoptosis and attenuating their accessional damages. These results suggested that ASP may serve as a novel potential antitumor agent in the future.
从黄芪中提取了醇溶性多糖（ASP），并研究了其初步的结构特征和体内抗肿瘤活性。ASP 中的总糖、蛋白质和糖醛酸含量分别为 92.04%、0.51%和 1.42%。傅里叶变换红外光谱（FTIR）和离子色谱（IC）结果表明，ASP（约 2.1×10 Da）是一种由阿拉伯糖、半乳糖、葡萄糖和甘露糖（摩尔比：1.00:0.98:3.01:1.52）组成的中性多糖，具有吡喃环和α型糖苷键。此外，ASP 能够通过提高血清细胞因子（TNF-α、IL-2 和 IFN-γ）和免疫细胞（巨噬细胞、淋巴细胞和 NK 细胞）的水平，显著抑制体内 H22 肝癌细胞的生长，从而诱导肿瘤细胞凋亡，减轻其继发损伤。这些结果表明，ASP 可能成为未来一种新型的潜在抗肿瘤药物。
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