Grup de Recerca GEMMAIR (AGAUR)- Medicina Aplicada, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d'Investigació Sanitària Pere Virgili (IISPV), Tarragona, Spain.
Servei de Medicina Interna, Hospital Universitari Joan XXIII, Tarragona, Spain.
PLoS One. 2018 Jul 16;13(7):e0200547. doi: 10.1371/journal.pone.0200547. eCollection 2018.
The aim of the study was to analyse the presence of several metabolites related to atherosclerosis in the plasma of patients with unstable carotid plaque and in the plasma of healthy subjects.
We included 20 patients who had undergone carotid endarterectomy and 20 healthy subjects as a control group. All the subjects recruited were male. We used a metabolomic approach with liquid chromatography coupled to mass spectrometry to evaluate plasma metabolite levels in the metabolic pathway involved in the progression of atherosclerotic plaque.
We observed that circulating levels of 20-HETE were significantly higher in patients with atheroma plaque than in healthy subjects (p = 0.018). No differences were found with regard to the other metabolites analysed. We also conducted a random forest analysis and found that 20-HETE was the main differentiator in the list of selected metabolites. In addition, plasma levels of 20-HETE correlated positively with body mass index (r = 0.427, p = 0.007) and diastolic blood pressure (r = 0.365, p = 0.028).
This study confirms that of all the molecules studied only 20-HETE is related to carotid plaque. Further studies are needed to compare patients with stable carotid plaque vs. patients with unstable carotid plaque in order to confirm that 20-HETE could be a potential factor related to carotid plaque.
本研究旨在分析不稳定颈动脉斑块患者的血浆中和健康受试者的血浆中与动脉粥样硬化相关的几种代谢物的存在情况。
我们纳入了 20 例行颈动脉内膜切除术的患者和 20 名健康受试者作为对照组。所有入组的受试者均为男性。我们采用液相色谱-质谱联用的代谢组学方法来评估参与动脉粥样硬化斑块进展的代谢途径中的血浆代谢物水平。
我们观察到，动脉粥样硬化斑块患者的循环 20-HETE 水平明显高于健康受试者（p = 0.018）。而对于其他分析的代谢物，我们没有发现差异。我们还进行了随机森林分析，发现 20-HETE 是所选代谢物列表中的主要区分物。此外，血浆 20-HETE 水平与体重指数呈正相关（r = 0.427，p = 0.007），与舒张压呈正相关（r = 0.365，p = 0.028）。
本研究证实，在所研究的所有分子中，只有 20-HETE 与颈动脉斑块有关。需要进一步研究以比较稳定颈动脉斑块患者与不稳定颈动脉斑块患者，以确认 20-HETE 可能是与颈动脉斑块相关的潜在因素。
