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牙干细胞的冷冻保存

Cryopreservation of Dental Stem Cells.

作者信息

Pilbauerová Nela, Suchánek Jakub

机构信息

Department of Dentistry, Charles University, Faculty of Medicine in Hradec Králové, and University Hospital, Hradec Králové, Czech Republic.

出版信息

Acta Medica (Hradec Kralove). 2018;61(1):1-7. doi: 10.14712/18059694.2018.16.

Abstract

Nowadays, regenerative and reparative medicine has grown in popularity. Dental stem cells are easily accessible source of adult stem cells. They can be harvested by a tooth extraction or spontaneous deciduous tooth exfoliation. They have to be isolated, expanded and stored until time they would be needed for individual stem cell therapy. Cryopreservation is both a short-term and long-term storage of tissues or cells at sub-zero temperatures. There are several methods of cryopreservation requiring different technologies. The objective of this review is to compare them and highlight their advantages and disadvantages.

摘要

如今,再生医学和修复医学越来越受欢迎。牙干细胞是成人干细胞的容易获取的来源。它们可以通过拔牙或乳牙自然脱落来采集。必须对它们进行分离、扩增并储存,直到需要用于个体干细胞治疗时。冷冻保存是在零下温度下对组织或细胞进行短期和长期储存。有几种冷冻保存方法,需要不同的技术。本综述的目的是对它们进行比较,并突出它们的优缺点。

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