Pilbauerová Nela, Suchánek Jakub
Department of Dentistry, Charles University, Faculty of Medicine in Hradec Králové, and University Hospital, Hradec Králové, Czech Republic.
Acta Medica (Hradec Kralove). 2018;61(1):1-7. doi: 10.14712/18059694.2018.16.
Nowadays, regenerative and reparative medicine has grown in popularity. Dental stem cells are easily accessible source of adult stem cells. They can be harvested by a tooth extraction or spontaneous deciduous tooth exfoliation. They have to be isolated, expanded and stored until time they would be needed for individual stem cell therapy. Cryopreservation is both a short-term and long-term storage of tissues or cells at sub-zero temperatures. There are several methods of cryopreservation requiring different technologies. The objective of this review is to compare them and highlight their advantages and disadvantages.
如今，再生医学和修复医学越来越受欢迎。牙干细胞是成人干细胞的容易获取的来源。它们可以通过拔牙或乳牙自然脱落来采集。必须对它们进行分离、扩增并储存，直到需要用于个体干细胞治疗时。冷冻保存是在零下温度下对组织或细胞进行短期和长期储存。有几种冷冻保存方法，需要不同的技术。本综述的目的是对它们进行比较，并突出它们的优缺点。
