Jacobs S K, Wilson D J, Kornblith P L, Grimm E A
J Neurosurg. 1986 Jan;64(1):114-7. doi: 10.3171/jns.1986.64.1.0114.
Culture of peripheral blood lymphocytes (PBL) from brain-tumor patients with recombinant interleukin-2 (IL-2) results in the activation of lymphokine-activated killer cells (LAK) with the capacity to lyse autologous and allogeneic glioblastoma. In this study, PBL obtained from brain-tumor patients were cultured with or without IL-2 for 3 to 7 days and then tested for their ability to lyse target cells in a 4-hour chromium release cytotoxicity assay. The PBL were drawn 1 to 2 weeks following operative tumor debulking. Cells used as targets included fresh brain-tumor cells obtained at the time of craniotomy, fresh brain-tumor cells grown from 1 to 3 weeks in tissue culture, fresh autologous PBL, and allogeneic glioblastoma cells grown in tissue culture. Peripheral blood lymphocytes from brain-tumor patients that were cultured without IL-2 did not significantly lyse autologous or allogeneic glioblastoma. However, when these PBL were cultured with IL-2, LAK were generated which produced marked lysis of autologous as well as allogeneic tissue-culture glioblastoma in all of eight cases. Significant lysis of autologous fresh tumor by patient LAK was observed in four of five experiments. By contrast, patient LAK did not kill autologous normal PBL. The ability to generate LAK was not influenced by the patient's age, previous therapy, or the administration of steroids.
用重组白细胞介素-2（IL-2）培养脑肿瘤患者的外周血淋巴细胞（PBL），可激活淋巴因子激活的杀伤细胞（LAK），这些细胞具有裂解自体和同种异体胶质母细胞瘤的能力。在本研究中，取自脑肿瘤患者的PBL在有或无IL-2的情况下培养3至7天，然后在4小时的铬释放细胞毒性试验中检测其裂解靶细胞的能力。PBL在肿瘤手术切除减瘤后1至2周采集。用作靶细胞的包括开颅时获得的新鲜脑肿瘤细胞、在组织培养中培养1至3周的新鲜脑肿瘤细胞、新鲜自体PBL以及在组织培养中生长的同种异体胶质母细胞瘤细胞。未用IL-2培养的脑肿瘤患者外周血淋巴细胞不会显著裂解自体或同种异体胶质母细胞瘤。然而，当这些PBL与IL-2一起培养时，会产生LAK，在所有8例病例中，这些LAK对自体和同种异体组织培养的胶质母细胞瘤均产生明显的裂解作用。在5个实验中的4个实验中观察到患者的LAK对自体新鲜肿瘤有显著裂解作用。相比之下，患者的LAK不会杀死自体正常PBL。产生LAK的能力不受患者年龄、既往治疗或类固醇给药的影响。
