Zhang Qian, Yu Lei, Qin Dandan, Huang Rui, Jiang Xiaochen, Zou Chendan, Tang Qingchao, Chen Yinggang, Wang Guiyu, Wang Xishan, Gao Xu
Department of Colorectal Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150086, PR China; Colorectal Cancer Institute of Harbin Medical University, Harbin, Heilongjiang, 150086, PR China.
Department of Nephrology, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150086, PR China.
PLoS One. 2015 Mar 23;10(3):e0120698. doi: 10.1371/journal.pone.0120698. eCollection 2015.
MicroRNAs (miRNAs) are deregulated in a number of cancers including colorectal cancer. MiR-30c belongs to miR-30 family, and is involved in a variety of malignant diseases. In this study, we detected the expression of miR-30c in colon cancer cell lines and clinical colon cancer specimens. MiR-30c was shown to be dramatically down-regulated both in cell lines and cancer tissues. Additionally, miR-30c could inhibit cancer cell growth, migration and invasion in vitro. Consistently, stable over-expression of miR-30c inhibited the growth and lung metastasis of colon cancer cell xenografts in vivo. Furthermore, bioinformatics algorithm and luciferase reporter assay indicated ADAM19 as a direct target of miR-30c. Of interest, further experiments demonstrated that inhibition of ADAM19 by miR-30c partially mediated the anti-tumor effect of miR-30c. Overall, our study provides the new insight that miR-30c inhibited colon cancer cells via targeting ADAM19. Thus, miR-30c might serve as a promising therapeutic strategy for colon cancer treatment.
微小RNA（miRNA）在包括结直肠癌在内的多种癌症中表达失调。MiR-30c属于miR-30家族，参与多种恶性疾病。在本研究中，我们检测了miR-30c在结肠癌细胞系和临床结肠癌标本中的表达。结果显示，miR-30c在细胞系和癌组织中均显著下调。此外，miR-30c在体外可抑制癌细胞的生长、迁移和侵袭。同样，miR-30c的稳定过表达在体内抑制了结肠癌细胞异种移植瘤的生长和肺转移。此外，生物信息学算法和荧光素酶报告基因检测表明ADAM19是miR-30c的直接靶点。有趣的是，进一步的实验表明，miR-30c对ADAM19的抑制作用部分介导了miR-30c的抗肿瘤作用。总体而言，我们的研究提供了新的见解，即miR-30c通过靶向ADAM19抑制结肠癌细胞。因此，miR-30c可能成为一种有前景的结肠癌治疗策略。
