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1
SV40 vector with early gene replacement efficient in transducing exogenous DNA into mammalian cells.具有早期基因替换功能的SV40载体能够高效地将外源DNA导入哺乳动物细胞。
Nucleic Acids Res. 1985 Dec 9;13(23):8573-86. doi: 10.1093/nar/13.23.8573.
2
Expression and excretion of human fibroblast beta 1 interferon in monkey cells after transfection with a recombinant SV40 plasmid vector.
J Mol Appl Genet. 1982;1(5):385-94.
3
Efficient introduction of plasmid DNA into human hemopoietic cells by encapsidation in simian virus 40 pseudovirions.通过包装在猴病毒40假病毒中，将质粒DNA高效导入人造血细胞。
Proc Natl Acad Sci U S A. 1986 Sep;83(18):6925-9. doi: 10.1073/pnas.83.18.6925.
4
Efficient constitutive production of human fibroblast interferon by hamster cells transformed with the IFN-beta 1 gene fused to an SV40 early promoter.通过用与SV40早期启动子融合的IFN-β1基因转化的仓鼠细胞高效组成型生产人成纤维细胞干扰素。
DNA. 1984 Aug;3(4):297-308. doi: 10.1089/dna.1.1984.3.297.
5
Transient expression of murine interferon-alpha genes in mouse and monkey cells.
Gene. 1986;45(2):159-65. doi: 10.1016/0378-1119(86)90250-7.
6
A packaging system for SV40 vectors without viral coding sequences.一种用于无病毒编码序列的SV40载体的包装系统。
Anal Biochem. 1997 Dec 1;254(1):139-43. doi: 10.1006/abio.1997.2417.
7
Conversion of a single-stranded simian virus 40 (SV40)-based shuttle vector to its double-stranded form does not require the SV40 T antigen in monkey cells.将基于单链猿猴病毒40（SV40）的穿梭载体转化为双链形式，在猴细胞中并不需要SV40 T抗原。
J Gen Virol. 1991 Dec;72 ( Pt 12):3091-3. doi: 10.1099/0022-1317-72-12-3091.
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Hepatitis B virus enhances transduction of human hepatocytes by SV40-based vectors.乙型肝炎病毒增强基于SV40的载体对人肝细胞的转导。
J Hepatol. 2004 Mar;40(3):520-6. doi: 10.1016/j.jhep.2003.11.028.
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SV40-based Escherichia coli shuttle vectors infectious for monkey cells.基于SV40的对猴细胞具有感染性的大肠杆菌穿梭载体。
Gene. 1987;53(1):21-9. doi: 10.1016/0378-1119(87)90089-8.
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Efficient in vivo encapsidation of a shuttle vector into pseudo-simian virus 40 virions using a shuttle virus as helper.利用穿梭病毒作为辅助，将穿梭载体高效地在体内包装到伪猴病毒40病毒粒子中。
J Gen Virol. 1992 Jun;73 ( Pt 6):1533-6. doi: 10.1099/0022-1317-73-6-1533.

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Cre Recombinase Mediates the Removal of Bacterial Backbone to Efficiently Generate rSV40.Cre重组酶介导细菌骨架的去除以高效生成重组猿猴病毒40（rSV40）。
Mol Ther Methods Clin Dev. 2018 Feb 27;9:225-233. doi: 10.1016/j.omtm.2018.02.010. eCollection 2018 Jun 15.

本文引用的文献

1
THE PURIFICATION OF SIMIAN VIRUS 40.猿猴病毒40的纯化
Virology. 1964 Nov;24:381-7. doi: 10.1016/0042-6822(64)90175-8.
2
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.用于比色法测定脱氧核糖核酸的二苯胺反应的条件及机制研究。
Biochem J. 1956 Feb;62(2):315-23. doi: 10.1042/bj0620315.
3
Infectious vaccinia virus recombinants that express hepatitis B virus surface antigen.表达乙型肝炎病毒表面抗原的感染性痘苗病毒重组体。
Nature. 1983 Apr 7;302(5908):490-5. doi: 10.1038/302490a0.
4
Structure and expression of a cloned cDNA for mouse interferon-beta.小鼠β干扰素克隆cDNA的结构与表达
J Biol Chem. 1983 Aug 10;258(15):9522-9.
5
Construction and applications of a highly transmissible murine retrovirus shuttle vector.一种高传染性小鼠逆转录病毒穿梭载体的构建与应用
Cell. 1984 Jul;37(3):1053-62. doi: 10.1016/0092-8674(84)90440-9.
6
Transcriptional 'enhancers' from SV40 and polyoma virus show a cell type preference.来自猴病毒40（SV40）和多瘤病毒的转录“增强子”表现出细胞类型偏好性。
Nucleic Acids Res. 1982 Dec 20;10(24):7965-76. doi: 10.1093/nar/10.24.7965.
7
Transcription of the simian virus 40 genome in DNA-transformed murine teratocarcinoma stem cells.猿猴病毒40基因组在DNA转化的小鼠畸胎瘤干细胞中的转录。
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6386-90. doi: 10.1073/pnas.78.10.6386.
8
Cell-surface expression of influenza haemagglutinin from a cloned DNA copy of the RNA gene.来自RNA基因克隆DNA拷贝的流感血凝素的细胞表面表达。
Nature. 1981 Oct 22;293(5834):620-5. doi: 10.1038/293620a0.
9
Transformation of mouse fibroblasts to methotrexate resistance by a recombinant plasmid expressing a prokaryotic dihydrofolate reductase.通过表达原核二氢叶酸还原酶的重组质粒将小鼠成纤维细胞转化为甲氨蝶呤抗性。
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1527-31. doi: 10.1073/pnas.78.3.1527.
10
Simian virus 40 tandem repeated sequences as an element of the early promoter.猿猴病毒40串联重复序列作为早期启动子的一个元件
Proc Natl Acad Sci U S A. 1981 Feb;78(2):943-7. doi: 10.1073/pnas.78.2.943.

具有早期基因替换功能的SV40载体能够高效地将外源DNA导入哺乳动物细胞。

SV40 vector with early gene replacement efficient in transducing exogenous DNA into mammalian cells.

作者信息

Asano M, Iwakura Y, Kawade Y

出版信息

Nucleic Acids Res. 1985 Dec 9;13(23):8573-86. doi: 10.1093/nar/13.23.8573.

DOI:10.1093/nar/13.23.8573
PMID:3001644
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC322153/
Abstract

An early replacement SV40 vector, SV40-Mu beta, was constructed by replacing part of the early genes of the virus with mouse interferon-beta (IFN-beta) cDNA. Upon transfection of COS-7 cells with this DNA, transducing viral particles were produced, which could infect various cells and cause efficient production of mouse IFN-beta. The viral stock contained no detectable wild-type SV40. The IFN production after the virus infection was very high in monkey kidney cells, but less so in human epithelial cells, and low in mouse, pig, hamster cells and in human lymphocytes. The efficiency of introduction of the DNA to monkey kidney cells was compared with that by the calcium phosphate precipitation method, and the viral vector was found to be more efficient by a factor of several tens to hundreds.

摘要

一种早期替代的SV40载体，即SV40-Muβ，是通过用小鼠干扰素-β（IFN-β）cDNA替换病毒的部分早期基因构建而成的。用这种DNA转染COS-7细胞后，产生了具有转导能力的病毒颗粒，这些颗粒能够感染各种细胞并高效产生小鼠IFN-β。病毒原液中未检测到野生型SV40。病毒感染后，猴肾细胞中IFN的产量非常高，但在人上皮细胞中较低，在小鼠、猪、仓鼠细胞和人淋巴细胞中产量很低。将该DNA导入猴肾细胞的效率与磷酸钙沉淀法进行了比较，发现病毒载体的效率要高几十到几百倍。