果蝇朱红眼基因座及其可抑制等位基因的分子特征分析。
Molecular characterization of the Drosophila vermilion locus and its suppressible alleles.
作者信息
Searles L L, Voelker R A
出版信息
Proc Natl Acad Sci U S A. 1986 Jan;83(2):404-8. doi: 10.1073/pnas.83.2.404.
We have cloned vermilion (v), one of the genes required for brown eye pigment synthesis in Drosophila, using a mutant (vH2a) "tagged" with the transposon P factor. Mutations that disrupt v gene expression are clustered within approximately 2 kilobases of DNA. A 1.4-kilobase transcript, homologous to this same region, is present in v+ RNA but absent in RNA from several v mutants. The spontaneous v alleles that are suppressed by the suppressor of sable [su(s)] are apparently identical insertions of 412, a copia-like transposable element. Preliminary evidence suggests that su(s)-suppressible alleles at other loci may also be 412 insertions.
我们利用被转座子P因子“标记”的突变体（vH2a）克隆了朱砂眼基因（v），该基因是果蝇棕色眼色素合成所需的基因之一。破坏v基因表达的突变集中在约2千碱基的DNA范围内。与该相同区域同源的一个1.4千碱基转录本存在于v + RNA中，但在几个v突变体的RNA中不存在。被黑貂色抑制因子[su（s）]抑制的自发v等位基因显然是类copia转座元件412的相同插入。初步证据表明，其他位点的su（s）可抑制等位基因也可能是412插入。
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