Baghban Roghayyeh, Farajnia Safar, Ghasemi Younes, Mortazavi Mojtaba, Zarghami Nosratollah, Samadi Naser
Medical Biotechnology Department, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran.
Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Curr Pharm Biotechnol. 2018;19(6):451-467. doi: 10.2174/1389201019666180718093037.
Although Pichia pastoris is an outstanding host among conventional expression systems for production of recombinant proteins, a new interest has been emerged to this system due to the inherent advantages and new developments in this expression host. The potential for secretory and soluble expression of heterologous glycoproteins in P. pastoris proposed this system as a candidate for the production of complex eukaryotic proteins.
Several new developments have occurred in different areas related to P. pastoris expression system including hosts, vectors, glycosylation pattern and fermentation technology. Strain engineering using Crispr/Cas9 technology to produce human-like glycoproteins and protease deficient strains are two new areas of development with high importance.
This review is dedicated to discuss the most important characteristics of P. pastoris with emphasis on new developments, especially in the field of glycoengineering, efficient expression vectors and promoters.
New developments that occurred in the P. pastoris expression system converted this system to a versatile host for the production of complex proteins. This progress paved the way for several proteins to enter the clinical trials or industrial processes with this valuable expression host.
尽管巴斯德毕赤酵母在传统重组蛋白表达系统中是一种出色的宿主，但由于该表达宿主的固有优势和新进展，人们对该系统产生了新的兴趣。巴斯德毕赤酵母分泌和可溶性表达异源糖蛋白的潜力使其成为生产复杂真核蛋白的候选系统。
与巴斯德毕赤酵母表达系统相关的不同领域出现了一些新进展，包括宿主、载体、糖基化模式和发酵技术。利用Crispr/Cas9技术生产类人糖蛋白和蛋白酶缺陷菌株的菌株工程是两个具有高度重要性的新发展领域。
本综述致力于讨论巴斯德毕赤酵母的最重要特征，重点是新进展，特别是在糖基工程、高效表达载体和启动子领域。
巴斯德毕赤酵母表达系统中出现的新进展使该系统成为生产复杂蛋白的通用宿主。这一进展为多种蛋白利用这个有价值的表达宿主进入临床试验或工业生产流程铺平了道路。
