School of Chemistry and Environment, South China Normal University, Higher Education Mega Center, Guangzhou 510006,People's Republic of China; Guangdong Engineering Technology Research Center for Drinking Water Safety, and Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, School of Chemistry and Environment, South China Normal University, Higher Education Mega Center, Guangzhou 510006, People's Republic of China.
School of Chemistry and Environment, South China Normal University, Higher Education Mega Center, Guangzhou 510006,People's Republic of China; Guangdong Engineering Technology Research Center for Drinking Water Safety, and Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, School of Chemistry and Environment, South China Normal University, Higher Education Mega Center, Guangzhou 510006, People's Republic of China.
Sci Total Environ. 2018 Dec 15;645:170-178. doi: 10.1016/j.scitotenv.2018.07.102. Epub 2018 Jul 17.
Sorptive sinks are extensively used in the bioaccessibility of organic contaminants, but their suitability for simulating the intestinal cell is seldom reported. In the present study, the sorption efficiency of PAHs by sorptive sinks including silica, poly(ethylene-co-vinyl acetate) (polyE), tenax, and C18 were compared with that by caco-2 cells. The elimination rate constants of phenanthrene, fluoranthene, pyrene, benzo(a)pyrene by caco-2 cell were 0.0417 ± 0.006 min, 0.0411 ± 0.0074 min, 0.0362 ± 0.006 min, and 0.0526 ± 0.0037 min, respectively, which were more closely to that of silica and polyE compared to other materials. This indicated that these materials might be the preferable sorptive sinks to simulate absorption of PAHs by intestinal cells. The bioaccessibility of phenanthrene, fluoranthene, pyrene, benzo(a)pyrene in indoor dust ranged from 15.5-43.5%, 9.10-38.8%, 10.0-37.9%, and 6.00-21.9%, respectively, based on physiologically based extraction test (PBET) and the sorptive sinks added in the intestinal solution led to 1.17 to 8.47-fold enhancement of bioaccessibility. The correlation of in vivo PAHs relative bioavailability (RBA) and in vitro digestion bioaccessibility with or without the sorptive sinks of indoor dust were measured, and the results indicated that silica and polyE were more likely to predict PAHs RBA of indoor dust, which was consistent with the results of sorption kinetics assay. The present results indicate that silica and polyE have the potential to simulate caco-2 cell and the inclusion of these materials in the PBET is likely to predict PAHs RBA in indoor dust. Capsule: Silica and polyE were more likely to simulate absorption of PAHs by intestinal cells, and to predict PAHs RBA of indoor dust.
吸附性容器广泛应用于有机污染物的生物可利用性研究，但很少有报道其模拟肠道细胞的适用性。本研究比较了吸附性容器（包括硅胶、聚乙烯-醋酸乙烯共聚物（polyE）、Tenax 和 C18）对多环芳烃（PAHs）的吸附效率与 Caco-2 细胞的吸附效率。Caco-2 细胞对菲、荧蒽、芘和苯并[a]芘的消除率常数分别为 0.0417±0.006 min、0.0411±0.0074 min、0.0362±0.006 min 和 0.0526±0.0037 min，与硅胶和 polyE 相比，这些值更接近其他材料。这表明这些材料可能是模拟肠道细胞吸收 PAHs 的更合适的吸附性容器。基于生理相关提取测试（PBET），室内灰尘中菲、荧蒽、芘和苯并[a]芘的生物可利用性分别为 15.5-43.5%、9.10-38.8%、10.0-37.9%和 6.00-21.9%，在肠道溶液中添加吸附性容器可使生物可利用性提高 1.17 至 8.47 倍。测量了体内多环芳烃相对生物利用度（RBA）和有无室内灰尘吸附性容器的体外消化生物可利用性的相关性，结果表明，硅胶和 polyE 更有可能预测室内灰尘中 PAHs 的 RBA，这与吸附动力学测定的结果一致。本研究结果表明，硅胶和 polyE 具有模拟 Caco-2 细胞的潜力，在 PBET 中包含这些材料可能有助于预测室内灰尘中 PAHs 的 RBA。
